|
| Status |
Public on Sep 17, 2015 |
| Title |
T20 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse embryonic fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
strain background: ICR
cell type: Mouse embryonic fibroblast
passages: 3-5
infected with: Oct4 Klf4 and Sox2
treated with: LSD1 inhibitor Tranylcypromine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
OSK treated with LSD1 inhibitor Tranylcypromine
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Sep 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Hao Sun |
| E-mail(s) |
sun_hao@gibh.ac.cn
|
| Organization name |
Chinese Academy of Sciences
|
| Lab |
Zheng's Lab
|
| Street address |
190 Kaiyuan Avenue
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN03076334 |
| SRA |
SRX708972 |
