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Sample GSM1511383 Query DataSets for GSM1511383
Status Public on Sep 17, 2015
Title MEF
Sample type SRA
Source name Mouse embryonic fibroblast
Organism Mus musculus
Characteristics strain background: ICR
cell type: Mouse embryonic fibroblast
passages: 3-5
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina MiSeq
Data processing Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: mm10
Supplementary_files_format_and_content: TPM
Submission date Sep 23, 2014
Last update date May 15, 2019
Contact name Hao Sun
Organization name Chinese Academy of Sciences
Lab Zheng's Lab
Street address 190 Kaiyuan Avenue
City Guangzhou
ZIP/Postal code 510530
Country China
Platform ID GPL16417
Series (1)
BioSample SAMN03076330
SRA SRX708968

Supplementary file Size Download File type/resource
GSM1511383_mef.genes.txt.gz 599.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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