|
Status |
Public on Sep 17, 2015 |
Title |
MEF |
Sample type |
SRA |
|
|
Source name |
Mouse embryonic fibroblast
|
Organism |
Mus musculus |
Characteristics |
strain background: ICR cell type: Mouse embryonic fibroblast passages: 3-5
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina MiSeq |
|
|
Data processing |
Illumina Casava1.7 software used for basecalling. Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie Transcripts Per Million were calculated using a protocol from RSEM Genome_build: mm10 Supplementary_files_format_and_content: TPM
|
|
|
Submission date |
Sep 23, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Hao Sun |
E-mail(s) |
sun_hao@gibh.ac.cn
|
Organization name |
Chinese Academy of Sciences
|
Lab |
Zheng's Lab
|
Street address |
190 Kaiyuan Avenue
|
City |
Guangzhou |
ZIP/Postal code |
510530 |
Country |
China |
|
|
Platform ID |
GPL16417 |
Series (1) |
|
Relations |
BioSample |
SAMN03076330 |
SRA |
SRX708968 |