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Series GSE61130 Query DataSets for GSE61130
Status Public on Jul 07, 2015
Title Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We expressed either a wt or a phosphomutant version of ZFP36L1 in IMR90 ER:RAS cells. 7 days upon RAS induction (when the cells reach a fully senescent phenotype) we collected the RNA. ZFP36L1 is a RNA binding protein that binds to AU-rich elements in the 3’UTR of mRNAs and triggers their degradation. Our previous experiments showed that the activity of ZFP36L1 was key in the regulation of the senescent phenotype.By performing RNAseq we have uncovered the effect of expressing a constitutively active mutant of ZFP36L1 within the senescent transcriptome.
Overall design 4 samples examined: Non-senescent cells (EV - 4OHT), Senescent cells (EV + 4OHT), Senescent cells expressing ZFP36L1wt and Senescent cells expressing ZFP36L1mut
Contributor(s) Herranz N, Gil J, Dharmalingam G
Citation(s) 26280535
Submission date Sep 04, 2014
Last update date May 15, 2019
Contact name Gopuraja Dharmalingam
Organization name MRC London Institute of Medical Sciences
Department Epigenetics section
Street address Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
City London
ZIP/Postal code W12 0NN
Country United Kingdom
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (12)
GSM1498119 EV - 4OHT Rep1
GSM1498120 EV + 4OHT Rep1
GSM1498121 ZFP36L1wt +4OHT Rep1
BioProject PRJNA260300
SRA SRP046254

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Supplementary file Size Download File type/resource
GSE61130_FPKM_BRF1_RNASeq.xlsx 6.4 Mb (ftp)(http) XLSX
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