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Sample GSM1498120 Query DataSets for GSM1498120
Status Public on Jul 07, 2015
Title EV + 4OHT Rep1
Sample type SRA
 
Source name IRM90 ER:RAS
Organism Homo sapiens
Characteristics cell line: IRM90
days after 4oht induction: 7
passage: p15
cell type: senescent cells
Treatment protocol To induce RAS expression, cells were treated with 4OHT [100nM]
Growth protocol IRM90 ER:RAS were grown in DMEM 10% FBS for 7 days. Media was changed at Day 4
Extracted molecule total RNA
Extraction protocol Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description Expression profile of senescent cells (EV + 4OHT)
Data processing RNA-Seq reads were aligned to human genome hg19 using Tophat verion 2.0.8 in strand specific mode. Gene annotations were obtained from Ensembl gene version 72. FPKM values were computed using R script
Genome_build: hg19
Supplementary_files_format_and_content: FPKM
 
Submission date Sep 04, 2014
Last update date May 15, 2019
Contact name Gopuraja Dharmalingam
Organization name MRC London Institute of Medical Sciences
Department Epigenetics section
Street address Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
City London
ZIP/Postal code W12 0NN
Country United Kingdom
 
Platform ID GPL16791
Series (1)
GSE61130 Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant
Relations
BioSample SAMN03019912
SRA SRX694427

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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