|
| Status |
Public on Jul 07, 2015 |
| Title |
EV + 4OHT Rep3 |
| Sample type |
SRA |
| |
|
| Source name |
IRM90 ER:RAS
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IRM90
days after 4oht induction: 7
passage: p15
cell type: senescent cells
|
| Treatment protocol |
To induce RAS expression, cells were treated with 4OHT [100nM]
|
| Growth protocol |
IRM90 ER:RAS were grown in DMEM 10% FBS for 7 days. Media was changed at Day 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Expression profile of senescent cells (EV + 4OHT)
|
| Data processing |
RNA-Seq reads were aligned to human genome hg19 using Tophat verion 2.0.8 in strand specific mode. Gene annotations were obtained from Ensembl gene version 72. FPKM values were computed using R script
Genome_build: hg19
Supplementary_files_format_and_content: FPKM
|
| |
|
| Submission date |
Sep 04, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Gopuraja Dharmalingam |
| Organization name |
MRC London Institute of Medical Sciences
|
| Department |
Epigenetics section
|
| Street address |
Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
|
| City |
London |
| ZIP/Postal code |
W12 0NN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL16791 |
| Series (1) |
| GSE61130 |
Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant |
|
| Relations |
| BioSample |
SAMN03019920 |
| SRA |
SRX694435 |
