|
Status |
Public on Jul 07, 2015 |
Title |
ZFP36L1wt +4OHT Rep1 |
Sample type |
SRA |
|
|
Source name |
IRM90 ER:RAS
|
Organism |
Homo sapiens |
Characteristics |
cell line: IRM90 days after 4oht induction: 7 passage: p15 cell type: senescent cells expressing ZFP36L1wt
|
Treatment protocol |
To induce RAS expression, cells were treated with 4OHT [100nM]
|
Growth protocol |
IRM90 ER:RAS were grown in DMEM 10% FBS for 7 days. Media was changed at Day 4
|
Extracted molecule |
total RNA |
Extraction protocol |
Libraries were prepared according to Illumina's kit instructions (mRNA stranded library prep kit)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
Expression profile of senescent cells expressing ZFP36L1wt
|
Data processing |
RNA-Seq reads were aligned to human genome hg19 using Tophat verion 2.0.8 in strand specific mode. Gene annotations were obtained from Ensembl gene version 72. FPKM values were computed using R script Genome_build: hg19 Supplementary_files_format_and_content: FPKM
|
|
|
Submission date |
Sep 04, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Gopuraja Dharmalingam |
Organization name |
MRC London Institute of Medical Sciences
|
Department |
Epigenetics section
|
Street address |
Faculty of Medicine, Imperial College, Hammersmith Hospital Campus
|
City |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE61130 |
Analysis of the senescent transcriptome upon expression of a ZFP36L1 phosphomutant |
|
Relations |
BioSample |
SAMN03019913 |
SRA |
SRX694428 |