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Series GSE59489 Query DataSets for GSE59489
Status Public on Aug 12, 2014
Title DNA methylation modifications associated with Chronic Fatigue Syndrome
Organism Homo sapiens
Experiment type Methylation profiling by genome tiling array
Summary Chronic Fatigue Syndrome (CFS), also known as myalgic encephalomyelitis, is a complex multifactorial disease that is characterized by the persistent presence of fatigue and other particular symptoms for a minimum of 6 months. Symptoms fail to dissipate after sufficient rest and have major effects on the daily functioning of CFS sufferers. CFS is a multi-system disease with a heterogeneous patient population showing a wide variety of functional disabilities and its biological basis remains poorly understood. Stable alterations in gene function in the immune system have been reported in several studies of CFS. Epigenetic modifications have been implicated in long-term effects on gene function, however, to our knowledge, genome-wide epigenetic modifications associated with CFS have not been explored. We examined the DNA methylome in peripheral blood mononuclear cells isolated from CFS patients and healthy controls using the Illumina HumanMethylation450 BeadChip array, controlling for invariant probes and probes overlapping polymorphic sequences.
Overall design Genomic DNA from 24 peripheral blood mononuclear cells (PBMC) samples (12 CFS, 12 controls) were bisulfite-converted and hybridised to the Illumina Infinium HumanMethylation450 BeadChip. GenomeStudio files were generated and the data was analyzed using the Illumina Methylation Analyzer R package.
Contributor(s) de Vega WC, Vernon SD, McGowan PO
Citation(s) 25111603
Submission date Jul 16, 2014
Last update date Mar 22, 2019
Contact name Patrick McGowan
Organization name University of Toronto Scarborough
Department Biological Sciences
Lab SW326
Street address 1265 Military Trail
City Toronto
State/province Ontario
ZIP/Postal code M1C 1A4
Country Canada
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (24)
GSM1437759 PBMC genomic DNA_CFS1
GSM1437760 PBMC genomic DNA_CFS2
GSM1437761 PBMC genomic DNA_CFS3
BioProject PRJNA255437

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59489_RAW.tar 183.1 Mb (http)(custom) TAR
GSE59489_non_normalized.txt.gz 58.5 Mb (ftp)(http) TXT
Processed data included within Sample table

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