GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE4277 Query DataSets for GSE4277
Status Public on Apr 29, 2009
Title IL1R2 and glucocorticoid responsiveness in patients with AIED
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Glucocorticoid responsive AIED patients demonstrate alternate splicing of IL1R2 in response to dexamethasone prior to treatment. Identification of IL1R2 as a potential target gene in AIED was determined by a microarray analysis of patients with end-stage AIED and controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation.
Overall design Patients with either end-stage AIED or controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation were enrolled. All patients received pneumovax 2 weeks prior to surgery. At the time of surgery, blood was taken for PBMC isolation and autologous perilymph was harvested from a bloodless field. For each patient, 3 microarrays were performed: (1) unstimulated PBMC, (2) pneumovax stimulated PBMC, (3) autologous perilymph stimulated PBMC. All conditions were compared for the AIED and control subjects. IL1R2 was identified as induced in controls, but not AIED patients, in response to autologous perilymph. Findings were confirmed by QPCR.
Contributor(s) Vambutas A, DeVoti J, Goldofsky E, Gordon M, Lesser M, Bonagura V
Citation(s) 19401759
Submission date Feb 20, 2006
Last update date Dec 06, 2018
Contact name Andrea Vambutas
Phone 718-470-7955
Fax 718-470-4281
Organization name Long Island Jewish Medical Center
Department Otolaryngology
Street address 430 Lakeville Road
City New Hyde Park
State/province NY
ZIP/Postal code 11042
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (18)
GSM97624 cochlear stim PBMC from AIED patient #1
GSM97625 pneumococcal stimulated PBMC from AIED patient #1
GSM97626 unstimulated PBMC from AIED patient #1
BioProject PRJNA94945

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4277_AIEDANOVAcorrectedanalysis.xls 40.2 Kb (ftp)(http) XLS
GSE4277_AIEDvsCTRLunfiltered.xls 58.1 Mb (ftp)(http) XLS

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap