|
| Status |
Public on Apr 29, 2009 |
| Title |
IL1R2 and glucocorticoid responsiveness in patients with AIED |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Glucocorticoid responsive AIED patients demonstrate alternate splicing of IL1R2 in response to dexamethasone prior to treatment. Identification of IL1R2 as a potential target gene in AIED was determined by a microarray analysis of patients with end-stage AIED and controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation.
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| |
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| Overall design |
Patients with either end-stage AIED or controls with non-immunologic etiologies of hearing loss undergoing cochlear implantation were enrolled. All patients received pneumovax 2 weeks prior to surgery. At the time of surgery, blood was taken for PBMC isolation and autologous perilymph was harvested from a bloodless field. For each patient, 3 microarrays were performed: (1) unstimulated PBMC, (2) pneumovax stimulated PBMC, (3) autologous perilymph stimulated PBMC. All conditions were compared for the AIED and control subjects. IL1R2 was identified as induced in controls, but not AIED patients, in response to autologous perilymph. Findings were confirmed by QPCR.
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| Contributor(s) |
Vambutas A, DeVoti J, Goldofsky E, Gordon M, Lesser M, Bonagura V |
| Citation(s) |
19401759 |
| |
| Submission date |
Feb 20, 2006 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Andrea Vambutas |
| E-mail(s) |
vambutas@lij.edu
|
| Phone |
718-470-7955
|
| Fax |
718-470-4281
|
| Organization name |
Long Island Jewish Medical Center
|
| Department |
Otolaryngology
|
| Street address |
430 Lakeville Road
|
| City |
New Hyde Park |
| State/province |
NY |
| ZIP/Postal code |
11042 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
|
| Samples (18)
|
| GSM97624 |
cochlear stim PBMC from AIED patient #1 |
| GSM97625 |
pneumococcal stimulated PBMC from AIED patient #1 |
| GSM97626 |
unstimulated PBMC from AIED patient #1 |
| GSM97627 |
cochlear perilymph stimulated PBMC of AIED patient #2 |
| GSM97629 |
pneumococcal stimulated PBMC, AIED patient #2 |
| GSM97630 |
unstimulated PBMC from AIED patient #2 |
| GSM97631 |
cochlear stimulated PBMC from AIED patient #3 |
| GSM97633 |
PBMC stimulated with pneumococcal antigen, AIED patient #3 |
| GSM97634 |
PBMC from AIED patient #3 |
| GSM97635 |
cochlear stimulated PBMC control patient #1 |
| GSM97636 |
cochlear stimulated PBMC from control #2 |
| GSM97637 |
cochlear stimulated PBMC from control #3 |
| GSM97638 |
pneumococcal stimulated PBMC of control patient #1 |
| GSM97640 |
pneumococcal stimulated PBMC from control patient #2 |
| GSM97641 |
pneumococcal stimulated PBMC from control #3 |
| GSM97643 |
unstimulated PBMC from control #1 |
| GSM97644 |
unstimulated PBMC control #2 |
| GSM97646 |
unstimulated PBMC from control patient #3 |
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| Relations |
| BioProject |
PRJNA94945 |
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