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| Status |
Public on Dec 01, 2012 |
| Title |
Gene expression signatures in a human bladder cancer cell line SW780 transfected with miR-137 precursor molecule |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To clarify the effect of miRNAs, we carried out a gene expression microarray analysis of SW780 cells transfected with a miR-137 precursor or a negative control. We found that 1,326 probe sets (1,016 unique genes) were downregulated (>2-fold) by ectopic miR-137 expression, including the previously reported miR-137 target genes CDK6, CDC42 and AURKA. Moreover, Gene Ontology analysis revealed that genes related to the cell cycle were significantly enriched among the affected genes.
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| Overall design |
SW780 cells were transfected with a Pre-miR-137 miRNA Precursor Molecule (Ambion) or Pre-miR miRNA Molecules Negative Control #1 (Ambion). Forty-eight hours after transfection, total RNA extraction was carried out, and gene expression signatures were analyzed.
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| Contributor(s) |
Suzuki H, Shimizu T |
| Citation(s) |
23200812 |
| Submission date |
Oct 22, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
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| Phone |
+81-11-611-2111
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| Organization name |
Sapporo Medical University
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| Department |
Department of Molecular Biology
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| Street address |
S1, W17, Chuo-ku
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| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA178119 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41760_RAW.tar |
29.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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