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Sample GSM1023652 Query DataSets for GSM1023652
Status Public on Dec 01, 2012
Title SW780 cells with miR-137 precursor molecule
Sample type RNA
Source name SW780
Organism Homo sapiens
Characteristics cell line: SW780
transfection: miR-137
Treatment protocol SW780 cells (3.0E+5 cells) were transfected with 100 pmol of a Pre-miR-137 miRNA Precursor Molecule (Ambion) or a Pre-miR miRNA Molecule Negative Control #1 (Ambion) using Lipofectamine 2000 according to the manufacturer’s instructions. Forty-eight hours after transfection, total RNA extraction was carried out.
Growth protocol SW780 cells were cultured in Leibovitz's L-15 medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37 ºC in a humidified incubator with 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using a TRIZOL reagent (Invitrogen, Carlsbad, CA).
Label Cy3
Label protocol Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
Hybridization protocol Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
Scan protocol Array was scanned with an Agilent G2565BA Microarray Scanner.
Description gene expression in SW780 cells transfected with miR-137 precursor molecule
Data processing Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
Submission date Oct 22, 2012
Last update date Dec 01, 2012
Contact name Hiromu Suzuki
Phone +81-11-611-2111
Organization name Sapporo Medical University
Department Department of Molecular Biology
Street address S1, W17, Chuo-ku
City Sapporo
State/province Hokkaido
ZIP/Postal code 060-8556
Country Japan
Platform ID GPL13607
Series (1)
GSE41760 Gene expression signatures in a human bladder cancer cell line SW780 transfected with miR-137 precursor molecule

Data table header descriptions
VALUE Background subtracted signal

Data table
1 2.65E+04
2 5.14E+00
3 5.19E+00
4 2.37E+02
5 2.76E+02
6 5.58E+01
7 3.96E+03
8 3.88E+02
9 5.41E+00
10 1.02E+02
11 5.47E+00
12 1.44E+03
13 1.88E+03
14 2.78E+02
15 1.37E+03
16 5.56E+00
17 1.45E+02
18 5.58E+00
19 5.59E+00
20 8.21E+02

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM1023652_SW780_mir-137.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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