|
| Status |
Public on Dec 01, 2012 |
| Title |
SW780 cells with control molecule |
| Sample type |
RNA |
| |
|
| Source name |
SW780
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SW780
transfection: control miRNA
|
| Treatment protocol |
SW780 cells (3.0E+5 cells) were transfected with 100 pmol of a Pre-miR-137 miRNA Precursor Molecule (Ambion) or a Pre-miR miRNA Molecule Negative Control #1 (Ambion) using Lipofectamine 2000 according to the manufacturer’s instructions. Forty-eight hours after transfection, total RNA extraction was carried out.
|
| Growth protocol |
SW780 cells were cultured in Leibovitz's L-15 medium supplemented with 10% heat-inactivated fetal bovine serum. The cultures were incubated at 37 ºC in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a TRIZOL reagent (Invitrogen, Carlsbad, CA).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
gene expression in SW780 cells transfected with control miRNA
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
| |
|
| Submission date |
Oct 22, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Hiromu Suzuki |
| E-mail(s) |
hsuzuki@sapmed.ac.jp
|
| Phone |
+81-11-611-2111
|
| Organization name |
Sapporo Medical University
|
| Department |
Department of Molecular Biology
|
| Street address |
S1, W17, Chuo-ku
|
| City |
Sapporo |
| State/province |
Hokkaido |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE41760 |
Gene expression signatures in a human bladder cancer cell line SW780 transfected with miR-137 precursor molecule |
|
