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| Status |
Public on Apr 05, 2013 |
| Title |
Systems-wide RNAi analysis of different chr13 genes in colorectal cancer cell line (SW480) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Expression profiles were generated for eight genes located on chromosome 13 following RNAi to enable the determination of gene specific LOF RNAi signatures in the colorectal cancer cell line SW480.
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| Overall design |
Triplicate transfections of SW480 cells were performed using two different siRNA duplexes corresponding to each gene target in 6-well plates using the same protocol scaled 30-fold. RNA was purified 72 hrs after transfection. RNA was also purified from negative control siRNA-transfected cells (AllStar siNegative (siNEG), Qiagen). Reduction in target mRNA levels was confirmed by real-time PCR prior to the array analysis.
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| Contributor(s) |
Camps J, Pitt JJ, Emons G, Hummons AB, Case CM, Grade M, Jones TL, Nguyen QT, Ghadimi BM, Beissbarth T, Difilippantonio MJ, Caplen NJ, Ried T |
| Citation(s) |
23319804 |
| |
| Submission date |
Nov 18, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Tim Beissbarth |
| E-mail(s) |
tim.beissbarth@ams.med.uni-goettingen.de
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| Organization name |
University Medical Center Göttingen
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| Department |
Medical Statistics
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| Street address |
Humboldtallee 32
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| City |
Göttingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (57)
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| Relations |
| BioProject |
PRJNA148193 |
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