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Sample GSM837376 Query DataSets for GSM837376
Status Public on Apr 05, 2013
Title POLR1D.3.3
Sample type RNA
 
Source name SW480; Transf2.3.POLR1D.3
Organism Homo sapiens
Characteristics cell line: SW480
cell type: colorectal cancer cells
sirna: POLR1D
sirna no.: 3
rep. no.: 3
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
Label Cy3
Label protocol Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
 
Hybridization protocol Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65C for 17 hours.
Scan protocol Agilent G2565BA
Description aneuploid, colon adenocarcinoma cell line (SW480), transfected with siRNA POLR1D
Data processing log2 transformation of signal intensity, quantile normalization
 
Submission date Nov 18, 2011
Last update date Apr 05, 2013
Contact name Tim Beissbarth
E-mail(s) tim.beissbarth@ams.med.uni-goettingen.de
Organization name University Medical Center Göttingen
Department Medical Statistics
Street address Humboldtallee 32
City Göttingen
ZIP/Postal code 37073
Country Germany
 
Platform ID GPL4133
Series (1)
GSE33824 Systems-wide RNAi analysis of different chr13 genes in colorectal cancer cell line (SW480)

Data table header descriptions
ID_REF
VALUE quantile normalized log2 signal intensity

Data table
ID_REF VALUE
1 15.7379565306167
2 5.54624737212787
3 5.66014028126157
4 5.61397143060219
5 5.59479204140529
6 5.51652410464075
7 5.67353310940064
8 5.59479204140529
9 5.58089882198736
10 5.54624737212787
11 5.58089882198736
12 11.2615271364803
13 6.42018739921348
14 9.62731220185048
15 6.081846855567
16 13.7543037280421
17 5.8762651487539
18 8.4457984963579
19 16.268033859917
20 6.06915938214728

Total number of rows: 45015

Table truncated, full table size 995 Kbytes.




Supplementary file Size Download File type/resource
GSM837376_US45102909_251485028563_S01_GE1-v5_95_Feb07_1_2.txt.gz 8.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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