|
| Status |
Public on Apr 05, 2013 |
| Title |
KLF5.3.1 |
| Sample type |
RNA |
| |
|
| Source name |
SW480; Transf1.1.KLF5.3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SW480
cell type: colorectal cancer cells
sirna: KLF5
sirna no.: 3
rep. no.: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
|
| |
|
| Hybridization protocol |
Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65C for 17 hours.
|
| Scan protocol |
Agilent G2565BA
|
| Description |
aneuploid, colon adenocarcinoma cell line (SW480), transfected with siRNA KLF5
|
| Data processing |
log2 transformation of signal intensity, quantile normalization
|
| |
|
| Submission date |
Nov 18, 2011 |
| Last update date |
Apr 05, 2013 |
| Contact name |
Tim Beissbarth |
| E-mail(s) |
tim.beissbarth@ams.med.uni-goettingen.de
|
| Organization name |
University Medical Center Göttingen
|
| Department |
Medical Statistics
|
| Street address |
Humboldtallee 32
|
| City |
Göttingen |
| ZIP/Postal code |
37073 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4133 |
| Series (1) |
| GSE33824 |
Systems-wide RNAi analysis of different chr13 genes in colorectal cancer cell line (SW480) |
|
