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Status |
Public on Apr 05, 2013 |
Title |
DIS3.1.1 |
Sample type |
RNA |
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|
Source name |
SW480; Transf2.1.DIS3.1
|
Organism |
Homo sapiens |
Characteristics |
cell line: SW480 cell type: colorectal cancer cells sirna: DIS3 sirna no.: 1 rep. no.: 1
|
Extracted molecule |
total RNA |
Extraction protocol |
RNeasy Mini Kit (Qiagen) + RNase-Free DNase Set (Qiagen)
|
Label |
Cy3
|
Label protocol |
Quick Amp Labeling Kit (Agilent Technologies, Cat. #: 5190-0442), including MMLV-RT and oligo dT-Promoter primers for cDNA sinthesis, and T7 RNA Polymerase, Cy3-CTP and NTPs for labeling.
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|
|
Hybridization protocol |
Agilent Gene Expression Hybridization Kit (Agilent Technologies, Cat. #: 5188-5242). Hybridizations were performed on SureHyb chambers on a rotator oven at 65C for 17 hours.
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Scan protocol |
Agilent G2565BA
|
Description |
aneuploid, colon adenocarcinoma cell line (SW480), transfected with siRNA DIS3
|
Data processing |
log2 transformation of signal intensity, quantile normalization
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|
|
Submission date |
Nov 18, 2011 |
Last update date |
Apr 05, 2013 |
Contact name |
Tim Beissbarth |
E-mail(s) |
tim.beissbarth@ams.med.uni-goettingen.de
|
Organization name |
University Medical Center Göttingen
|
Department |
Medical Statistics
|
Street address |
Humboldtallee 32
|
City |
Göttingen |
ZIP/Postal code |
37073 |
Country |
Germany |
|
|
Platform ID |
GPL4133 |
Series (1) |
GSE33824 |
Systems-wide RNAi analysis of different chr13 genes in colorectal cancer cell line (SW480) |
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