|
| Status |
Public on May 22, 2024 |
| Title |
Terminal nucleotidyl transferases govern secondary siRNA production at distinct steps in plants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
In this study, we aim at understanding the contributions from TUTases, HESO1 and URT1, in miRNA-targeted secondary siRNA production in Arabidopsis thaliana. We have sequenced small RNAs from various T-DNA insertation single/double/triple mutants and compared their capability of producing ectopic secondary siRNAs from endogenous miRNA targets. The DESeq analysis is also included.
|
| |
|
| Overall design |
small RNA seq data from mutant lines (ski2-5, rrp4-2, urt1-1, heso1-4, heso1-1/urt1-1, rrp4-2/urt1-1/heso1-4, rrp4-2/urt1-1/dcl2CRISPR) and a wild type line. All lines are ecotype Col-0. There are three biological triplicates of each sample. The tissue that has been sequences is inflorescences.
|
| |
|
| Contributor(s) |
Vigh ML, Thieffry A, Arribas-Hernández L, Brodersen P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 20, 2024 |
| Last update date |
May 22, 2024 |
| Contact name |
Peter Brodersen |
| E-mail(s) |
pbrodersen@bio.ku.dk
|
| Organization name |
University of Copenhagen
|
| Street address |
Ole Maaløes Vej 5
|
| City |
København N |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platforms (1) |
| GPL19580 |
Illumina NextSeq 500 (Arabidopsis thaliana) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA1113689 |
Less...
More...