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| Status |
Public on Jun 24, 2019 |
| Title |
A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
| Organism |
Saccharomyces cerevisiae W303 |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Ribosome biogenesis is a complex and energy-demanding process requiring tight coordination of ribosomal RNA (rRNA) and ribosomal protein (RP) production. Alteration of any step in this process may impact growth by leading to proteotoxic stress. Although the transcription factor Hsf1 has emerged as a central regulator of proteostasis, how its activity is coordinated with ribosome biogenesis is unknown. Here we show that arrest of ribosome biogenesis in the budding yeast S. cerevisiae triggers rapid activation of a highly specific stress pathway that coordinately up-regulates Hsf1 target genes and down-regulates RP genes. Activation of Hsf1 target genes requires neo-synthesis of RPs, which accumulate in an insoluble fraction, leading to sequestration of the RP transcriptional activator Ifh1. Our data suggest that levels of newly-synthetized RPs, imported into the nucleus but not yet assembled into ribosomes, work to continuously balance Hsf1 and Ifh1 activity, thus guarding against proteotoxic stress during ribosome assembly.
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| Overall design |
RNA Polymerase II binding was measured upon nuclear depletion of topoisomerase 1, 2 or both and/or following cycloheximide, diazaborine or rapamycin treatment.
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| Contributor(s) |
Albert B, Shore D |
| Citation(s) |
31124783, 35768402 |
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| Submission date |
Jan 17, 2019 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Benjamin Albert |
| E-mail(s) |
benjamin.albert@unige.ch
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| Organization name |
University of Genevea
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| Department |
Bimol
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| Lab |
David Shore
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| Street address |
30, quai Ernest-Ansermet
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| City |
GENEVA |
| ZIP/Postal code |
CH-1211 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL25517 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae W303) |
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| Samples (63)
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| Relations |
| BioProject |
PRJNA515604 |
| SRA |
SRP179920 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125226_RAW.tar |
2.4 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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