|
| Status |
Public on Jun 24, 2019 |
| Title |
Top1-AID HSF1-FRB _AuxRap |
| Sample type |
SRA |
| |
|
| Source name |
whole organism
|
| Organism |
Saccharomyces cerevisiae W303 |
| Characteristics |
strain: W303
genotype: W303 background;tor1-1 fpr1<delta>::Nat RPL13A-2xFKBP12-TRP1 HSF1-FRB-KanMX6 TOP1-AID-KanMX6 MAT[alpha]
treatment: Auxin+rapamycin
antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131)
time: 50min after treatment
|
| Growth protocol |
cells were grown at 30 degrees in YPAD to mid-log phase before treatment
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
TruSeq ChIP library preparation kit (Illumina)
ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
For ChIP-Seq raw reads were aligned to the sacCer3
For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference.
For ChIP-Seq, reads that mapped to both sacCer3
For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp
Genome_build: sacCer3
Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
|
| |
|
| Submission date |
Jan 17, 2019 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Benjamin Albert |
| E-mail(s) |
benjamin.albert@unige.ch
|
| Organization name |
University of Genevea
|
| Department |
Bimol
|
| Lab |
David Shore
|
| Street address |
30, quai Ernest-Ansermet
|
| City |
GENEVA |
| ZIP/Postal code |
CH-1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL25517 |
| Series (1) |
| GSE125226 |
A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
|
| Relations |
| BioSample |
SAMN10762439 |
| SRA |
SRX5257231 |
