|
Status |
Public on Jun 24, 2019 |
Title |
Top1-AID HSF1-FRB _Aux |
Sample type |
SRA |
|
|
Source name |
whole organism
|
Organism |
Saccharomyces cerevisiae W303 |
Characteristics |
strain: W303 genotype: W303 background;tor1-1 fpr1<delta>::Nat RPL13A-2xFKBP12-TRP1 HSF1-FRB-KanMX6 TOP1-AID-KanMX6 MAT[alpha] treatment: Auxin antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131) time: 50min after treatment
|
Growth protocol |
cells were grown at 30 degrees in YPAD to mid-log phase before treatment
|
Extracted molecule |
genomic DNA |
Extraction protocol |
TruSeq ChIP library preparation kit (Illumina) ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045).
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
For ChIP-Seq raw reads were aligned to the sacCer3 For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference. For ChIP-Seq, reads that mapped to both sacCer3 For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp Genome_build: sacCer3 Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
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|
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Submission date |
Jan 17, 2019 |
Last update date |
Jun 26, 2019 |
Contact name |
Benjamin Albert |
E-mail(s) |
benjamin.albert@unige.ch
|
Organization name |
University of Genevea
|
Department |
Bimol
|
Lab |
David Shore
|
Street address |
30, quai Ernest-Ansermet
|
City |
GENEVA |
ZIP/Postal code |
CH-1211 |
Country |
Switzerland |
|
|
Platform ID |
GPL25517 |
Series (1) |
GSE125226 |
A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
|
Relations |
BioSample |
SAMN10762440 |
SRA |
SRX5257230 |