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Status |
Public on Jun 24, 2019 |
Title |
Top1-AID 60min_Aux |
Sample type |
SRA |
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Source name |
whole organism
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Organism |
Saccharomyces cerevisiae W303 |
Characteristics |
strain: W303 genotype: W303 background;leu2-3,112 HIS3,15 ura3-1::pADH1-OsTIR1-URA3 ADE2 trp1-1 can1-100 TOP1-AID-KanMX6 MAT[alpha] treatment: auxin antibody: Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) (ab5131) time: 20min after treatment
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Growth protocol |
cells were grown at 30 degrees in YPAD to mid-log phase before treatment
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Extracted molecule |
genomic DNA |
Extraction protocol |
TruSeq ChIP library preparation kit (Illumina) ChIP-seq was performed as described previously (Ribaud et al., 2012) (PMID: 21952045).
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Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2500 |
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Data processing |
For ChIP-Seq raw reads were aligned to the sacCer3 For ChIP-Seq, data were filtered to retain only the mapped reads with at most 5 hits in the reference. For ChIP-Seq, reads that mapped to both sacCer3 For ChIP-Seq, the reads were shifted by 100 bp and extended by 50 bp Genome_build: sacCer3 Supplementary_files_format_and_content: wig and bigwig files were obtained using HTSStation (http://htsstation.epfl.ch)
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Submission date |
Jan 17, 2019 |
Last update date |
Jun 26, 2019 |
Contact name |
Benjamin Albert |
E-mail(s) |
benjamin.albert@unige.ch
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Organization name |
University of Genevea
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Department |
Bimol
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Lab |
David Shore
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Street address |
30, quai Ernest-Ansermet
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City |
GENEVA |
ZIP/Postal code |
CH-1211 |
Country |
Switzerland |
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Platform ID |
GPL25517 |
Series (1) |
GSE125226 |
A ribosome assembly stress response regulates transcription to maintain proteome homeostasis |
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Relations |
BioSample |
SAMN10762414 |
SRA |
SRX5257217 |
Supplementary file |
Size |
Download |
File type/resource |
GSM3566640_S6_Top1_T60_aux.bw |
35.8 Mb |
(ftp)(http) |
BW |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data provided as supplementary file |
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