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Series GSE112351 Query DataSets for GSE112351
Status Public on Nov 16, 2018
Title Dominant roles of B cells in the initiation of CD4 T cell response to a virus-derived nanoparticle antigen
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Nanoparticles, including virus-like particles (VLP), are attractive candidates as carriers for vaccines and drug delivery. However, little is known about how they are targeted to the immune system in vivo. Using RNA phage Qb-derived VLP (Qb-VLP) as a model antigen, we found surprisingly that antigen-specific B cells, instead of dendritic cells (DCs), were the dominant antigen presenting cells that initiate the naïve CD4 T cell activation, and were sufficient to induce T follicular helper cell development in the absence of DCs. Qb-specific B cells promoted CD4 T cell proliferation by providing cognate interactions and cell differentiation by generating cytokine milieu which depended on the Toll-like receptor signaling in B cells. Moreover, antigen-specific B cells were also involved in initiating CD4 T cell response to influenza virus. The mechanism revealed here will advance the rational design of nanoparticles as vaccine candidates, especially for therapeutic vaccines that aim to break immune tolerance.
Overall design We measured expression profiles by RNA-seq in naïve B cells and Qb-VLP-activated B cells including plasma cells (PC).
Contributor(s) Hong S, Zhang Z, Liu H, Tian M, Zhang Z, Zhu X, Wang W, Zhu B, Zhou X, Zhang F, Ge Q, Tang H, Hua Z, Hou B
Citation(s) 30291027
Submission date Mar 26, 2018
Last update date Mar 08, 2019
Contact name Zhuqiang Zhang
Organization name Institute of Biophysics, CAS
Street address 15 Datun Road, Chaoyang District.
City Beijing
State/province ---
ZIP/Postal code 100101
Country China
Platforms (2)
GPL21273 HiSeq X Ten (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (12)
GSM3068216 naive-B-cell-RNA.rep1
GSM3068217 naive-B-cell-RNA.rep2
GSM3068218 naive-B-cell-RNA.rep3
BioProject PRJNA445727
SRA SRP136492

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE112351_RAW.tar 5.3 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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