|
Status |
Public on Nov 16, 2018 |
Title |
day3-B-cell-RNA.rep3 |
Sample type |
SRA |
|
|
Source name |
antigen stimulated day3 B
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 cell type: primary lymphocytes stimulation: antigen stimulated day3 B cells genotype/variation: wild type tissue: spleen developmental stage: 8 weeks
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Total RNA from at least 50,000 isolated mouse B cells was extracted by Trizol (Life Technology) and precipitated by RNase-free glycogen(Life technology). Immediately, we isolated mRNA from total RNA with NEBNext® Poly(A) mRNA Magnetic isolation Module(E7490S). high throughput sequencing library was constructed with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®(E7770S).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Basecalls performed by Annoroad Co. Ltd. (naïve B and Day2 B) / BerryGenomics Co. Ltd. (Day3 B and day3 PCs) RNA-Seq data were mapped by STAR aligner v2.5.2b and quantitified by Cufflinks and Cuffdiff. Genome_build: GENCODE M12 (mm10) Supplementary_files_format_and_content: The transcription levels (RPKM) were quantified with Cufflinks (v2.0.2) software.
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|
|
Submission date |
Mar 26, 2018 |
Last update date |
Nov 16, 2018 |
Contact name |
Zhuqiang Zhang |
E-mail(s) |
zhangzhuqiang@ibp.ac.cn
|
Organization name |
Institute of Biophysics, CAS
|
Street address |
15 Datun Road, Chaoyang District.
|
City |
Beijing |
State/province |
--- |
ZIP/Postal code |
100101 |
Country |
China |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE112351 |
Dominant roles of B cells in the initiation of CD4 T cell response to a virus-derived nanoparticle antigen |
|
Relations |
BioSample |
SAMN08797464 |
SRA |
SRX3849282 |