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Sample GSM3068217 Query DataSets for GSM3068217
Status Public on Nov 16, 2018
Title naive-B-cell-RNA.rep2
Sample type SRA
 
Source name naive B cells
Organism Mus musculus
Characteristics strain background: C57BL/6
cell type: primary lymphocytes
stimulation: naive B cells
genotype/variation: wild type
tissue: spleen
developmental stage: 8 weeks
Extracted molecule polyA RNA
Extraction protocol Total RNA from at least 50,000 isolated mouse B cells was extracted by Trizol (Life Technology) and precipitated by RNase-free glycogen(Life technology). Immediately, we isolated mRNA from total RNA with NEBNext® Poly(A) mRNA Magnetic isolation Module(E7490S).
high throughput sequencing library was constructed with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®(E7770S).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Basecalls performed by Annoroad Co. Ltd. (naïve B and Day2 B) / BerryGenomics Co. Ltd. (Day3 B and day3 PCs)
RNA-Seq data were mapped by STAR aligner v2.5.2b and quantitified by Cufflinks and Cuffdiff.
Genome_build: GENCODE M12 (mm10)
Supplementary_files_format_and_content: The transcription levels (RPKM) were quantified with Cufflinks (v2.0.2) software.
 
Submission date Mar 26, 2018
Last update date Nov 16, 2018
Contact name Zhuqiang Zhang
E-mail(s) zhangzhuqiang@ibp.ac.cn
Organization name Institute of Biophysics, CAS
Street address 15 Datun Road, Chaoyang District.
City Beijing
State/province ---
ZIP/Postal code 100101
Country China
 
Platform ID GPL21273
Series (1)
GSE112351 Dominant roles of B cells in the initiation of CD4 T cell response to a virus-derived nanoparticle antigen
Relations
BioSample SAMN08797472
SRA SRX3849274

Supplementary file Size Download File type/resource
GSM3068217_naive-rep2.RPKM.table.txt.gz 448.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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