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Sample GSM3068226 Query DataSets for GSM3068226
Status Public on Nov 16, 2018
Title day3-PC-cell-RNA.rep2
Sample type SRA
Source name antigen stimulated day3 PCs
Organism Mus musculus
Characteristics strain background: C57BL/6
cell type: primary lymphocytes
stimulation: antigen stimulated day3 PCs
genotype/variation: wild type
tissue: spleen
developmental stage: 8 weeks
Extracted molecule polyA RNA
Extraction protocol Total RNA from at least 50,000 isolated mouse B cells was extracted by Trizol (Life Technology) and precipitated by RNase-free glycogen(Life technology). Immediately, we isolated mRNA from total RNA with NEBNext® Poly(A) mRNA Magnetic isolation Module(E7490S).
high throughput sequencing library was constructed with NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®(E7770S).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing Basecalls performed by Annoroad Co. Ltd. (naïve B and Day2 B) / BerryGenomics Co. Ltd. (Day3 B and day3 PCs)
RNA-Seq data were mapped by STAR aligner v2.5.2b and quantitified by Cufflinks and Cuffdiff.
Genome_build: GENCODE M12 (mm10)
Supplementary_files_format_and_content: The transcription levels (RPKM) were quantified with Cufflinks (v2.0.2) software.
Submission date Mar 26, 2018
Last update date Nov 16, 2018
Contact name Zhuqiang Zhang
Organization name Institute of Biophysics, CAS
Street address 15 Datun Road, Chaoyang District.
City Beijing
State/province ---
ZIP/Postal code 100101
Country China
Platform ID GPL24247
Series (1)
GSE112351 Dominant roles of B cells in the initiation of CD4 T cell response to a virus-derived nanoparticle antigen
BioSample SAMN08797461
SRA SRX3849284

Supplementary file Size Download File type/resource
GSM3068226_D3PC-rep2.RPKM.table.txt.gz 469.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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