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| Status |
Public on Mar 26, 2018 |
| Title |
RNA-seq analysis of gene expression profile in Uhrf1 WT and KO germinal center B cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The goals of this study are to compare gene expression profiles of Uhrf1 WT and KO germinal center B cells and reveal the underlying mechanisms by which Uhrf1 regulates germinal center B cell responses. We found that upon Uhrf1 depeletion in germinal center B cells, most of the differentially expressed genes were upreguated.
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| Overall design |
The germinal center B cell specific Uhrf1-deficient mice and WT control mice were immunized and 10e6 germinal center B cells were FACS sorted for RNA extraction. Library were then generated with illumina protocol and subject to NGS and bioinformatic analysis.
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| Contributor(s) |
Chen C, Wang X |
| Citation(s) |
29618490 |
| |
| Submission date |
Aug 04, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Xiaoming Wang |
| E-mail(s) |
15295795206@163.com
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| Organization name |
Nanjing Medical University
|
| Street address |
101 Longmian Road
|
| City |
Nanjing |
| ZIP/Postal code |
211166 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA397185 |
| SRA |
SRP114838 |
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