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Series GSE102270 Query DataSets for GSE102270
Status Public on Mar 26, 2018
Title RNA-seq analysis of gene expression profile in Uhrf1 WT and KO germinal center B cells
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goals of this study are to compare gene expression profiles of Uhrf1 WT and KO germinal center B cells and reveal the underlying mechanisms by which Uhrf1 regulates germinal center B cell responses. We found that upon Uhrf1 depeletion in germinal center B cells, most of the differentially expressed genes were upreguated.
Overall design The germinal center B cell specific Uhrf1-deficient mice and WT control mice were immunized and 10e6 germinal center B cells were FACS sorted for RNA extraction. Library were then generated with illumina protocol and subject to NGS and bioinformatic analysis.
Contributor(s) Chen C, Wang X
Citation(s) 29618490
Submission date Aug 04, 2017
Last update date Jul 25, 2021
Contact name Xiaoming Wang
Organization name Nanjing Medical University
Street address 101 Longmian Road
City Nanjing
ZIP/Postal code 211166
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM2732711 wt1
GSM2732712 wt2
GSM2732713 ko1
BioProject PRJNA397185
SRA SRP114838

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE102270_RAW.tar 1.6 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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