|
| Status |
Public on Mar 26, 2018 |
| Title |
wt1 |
| Sample type |
SRA |
| |
|
| Source name |
germinal center b cell
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: germinal center b cell
age: 6-10 weeks
genotype/variation: Uhrf1 wt
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Splenic germinal center B cells were FACS sorted, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v2.
Fragments Per Kilobase per Million mapped fragments (FPKM) were calculated using Cufflinks software.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
|
| |
|
| Submission date |
Aug 04, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Xiaoming Wang |
| E-mail(s) |
15295795206@163.com
|
| Organization name |
Nanjing Medical University
|
| Street address |
101 Longmian Road
|
| City |
Nanjing |
| ZIP/Postal code |
211166 |
| Country |
China |
| |
|
| Platform ID |
GPL21273 |
| Series (1) |
| GSE102270 |
RNA-seq analysis of gene expression profile in Uhrf1 WT and KO germinal center B cells |
|
| Relations |
| BioSample |
SAMN07450549 |
| SRA |
SRX3061858 |
