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Sample GSM2732712 Query DataSets for GSM2732712
Status Public on Mar 26, 2018
Title wt2
Sample type SRA
Source name germinal center b cell
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: germinal center b cell
age: 6-10 weeks
genotype/variation: Uhrf1 wt
Extracted molecule total RNA
Extraction protocol Splenic germinal center B cells were FACS sorted, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v2.
Fragments Per Kilobase per Million mapped fragments (FPKM) were calculated using Cufflinks software.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
Submission date Aug 04, 2017
Last update date May 15, 2019
Contact name Xiaoming Wang
Organization name Nanjing Medical University
Street address 101 Longmian Road
City Nanjing
ZIP/Postal code 211166
Country China
Platform ID GPL21273
Series (1)
GSE102270 RNA-seq analysis of gene expression profile in Uhrf1 WT and KO germinal center B cells
BioSample SAMN07450590
SRA SRX3061859

Supplementary file Size Download File type/resource
GSM2732712_wt2.FPKM.txt.gz 409.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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