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Sample GSM2732713 Query DataSets for GSM2732713
Status Public on Mar 26, 2018
Title ko1
Sample type SRA
 
Source name germinal center b cell
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: germinal center b cell
age: 6-10 weeks
genotype/variation: Uhrf1 fl/fl x AID-Cre+
Extracted molecule total RNA
Extraction protocol Splenic germinal center B cells were FACS sorted, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie v2.
Fragments Per Kilobase per Million mapped fragments (FPKM) were calculated using Cufflinks software.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for each Sample ...
 
Submission date Aug 04, 2017
Last update date May 15, 2019
Contact name Xiaoming Wang
E-mail(s) 15295795206@163.com
Organization name Nanjing Medical University
Street address 101 Longmian Road
City Nanjing
ZIP/Postal code 211166
Country China
 
Platform ID GPL21273
Series (1)
GSE102270 RNA-seq analysis of gene expression profile in Uhrf1 WT and KO germinal center B cells
Relations
BioSample SAMN07450585
SRA SRX3061860

Supplementary file Size Download File type/resource
GSM2732713_ko1.FPKM.txt.gz 421.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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