Elements for the flower-specific cDNA microarray were obtained from three different sources. First, for those genes that are known or suspected to be involved in flower development, we either amplified fragments of their transcribed regions from cDNA or, if available, obtained cDNA clones from EST clone collections. Second, we generated cDNA libraries using RNA extracted from different floral tissues. For all libraries, the RNA preparations were subtracted with leaf RNA to reduce the abundance of clones representing ubiquitously expressed genes and thus, to enrich for flower-specific transcripts. The identity of most of the library clones was not determined prior to their use for preparing the array. Lastly, we added a set of 2632 non-redundant clones from a non-subtracted flower-derived library. The array is comprised of 10,816 elements representing approximately 5000-6000 genes. Keywords = Flower Keywords = cDNA array
Quality control of PCR products; codes are explained in Wellmer et al. (2004), Plant Cell 16, 1314-1326
Vector Type
Vectors used for subcloning of cDNA fragments; further explained in Wellmer et al. (2004), Plant Cell 16, 1314-1326
Remarks
Contains mainly accession numbers for clones from a non-redundant flower derived cDNA library; further details in Wellmer et al. (2004), Plant Cell 16, 1314-1326
SPOT_ID
Data table
ID
CLONE_ID
Name
GB_ACC
SEQUENCE
Q-PCR
Vector Type
Remarks
SPOT_ID
GP000A1
At4g12550
putative cell wall-plasma membrane disconnecting CLCT protein (AIR1A)
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