GEO DataSets

(Submitter supplied) African swine fever (ASF) is a deadly disease of swine currently causing a worldwide pandemic leading to severe economic consequences for the porcine industry. The control of disease spread is hampered by the limitation of available effective vaccines. Live attenuated vaccines (LAVs) are currently the most advanced vaccine prototypes, providing strong protection against ASF. However, the significant advances achieved using LAVs must be complemented with further studies to analyse vaccine-induced immunity. more...

Organism:

Sus scrofa domesticus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29562

60 Samples

Download data: TXT

(Submitter supplied) N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Accumulating evidence has shown that m6A methylation plays a vital role in various biological processes. However, research on the role of m6A modifications in regulation of porcine muscle growth is lacking. In this study, we identified differentially expressed genes in the early stages of muscle development between Large White (LW) and NingXiang (NX) pigs and further reported m6A methylation patterns via MeRIP-seq. more...

Organism:

Sus scrofa

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL26351

12 Samples

Download data: TXT

(Submitter supplied) Background: Autosomal dominant polycystic kidney disease (PKD) is characterized by extensive cyst formation and progressive fibrosis. However, the molecular mechanisms whereby the loss/loss-of-function of polycystin 1 or 2 (PC1/2) provokes fibrosis are largely unknown. The small GTPase RhoA has been implicated in cystogenesis, while we have shown that the RhoA/cytoskeleton/myocardin-related transcription factor (MRTF) pathway is a key inducer of epithelium-induced fibrogenesis. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

20 Samples

Download data: TXT

(Submitter supplied) Cardiac injury triggered by early metabolic disease is poorly investigated due to the complexity of pathophysiology. We comprehensively reveal the cellular landscape of healthy and metabolically disturbed heart through single-nucleus RNA sequencing, using the metabolic disease-susceptible transgenic pig model as material.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22475

2 Samples

Download data: MTX, TSV

(Submitter supplied) Intramuscular fat (IMF) in pork holds significant importance for economic performance within the pig industry and dietary calcium supplementation enhances the accumulation of intramuscular fat. Additionally, calcium ions inhibit translation and reduce protein synthesis. However, the mechanism by which calcium regulates IMF deposition in muscle through translation remains largely unknown. In this study, we compared the ribosome profiles of the longissimus dorsi muscles of trigram pigs from the normal calcium (NC) group or calcium supplement (HC) group by Ribo-seq, and RNA-seq. more...

Organism:

Sus scrofa

Type:

Other; Expression profiling by high throughput sequencing

Platform:

GPL22918

12 Samples

Download data: BW

(Submitter supplied) The gut microbiota is closely associated with digestion, metabolism, immunity, and host health. The imbalance of the microbial community in livestock directly affects their well-being and, consequently, productivity. The composition and diversity of the gut microbiota are influenced not only by host genetics but also by environmental factors such as the microbial complexity of the rearing environment, feeds, and antibiotics. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20809

8 Samples

Download data: ZIP

(Submitter supplied) N6-methyladenosine (m6A), the most abundant mRNA modification, can regulate various mRNA metabolism to affect numerous physiological and pathophysiological processes, including immune function. However, the regulation of m6A methylation and its role in immune defense against virus infections remain unexplored. Here, we found that Porcine rotavirus (PoRV) infection decreased global m6A levels in host cells by downregulating m6A writer METTL3. more...

Organism:

Porcine rotavirus; Sus scrofa

Type:

Other

Platforms:

GPL34387 GPL22475

18 Samples

Download data: BW

(Submitter supplied) Spermatozoa acquired motility and matured in epididymis after production in testis; however, the characteristics of sperm development between species remain poorly understood. In this study, we constructed a single-cell RNA dataset to investigate spermatogenesis characteristics between mouse and pig. This approach enabled us to analyze cell compositions in both testicular and epididymal tissues, providing insights into changes occurring during meiosis and spermiogenesis in mouse and pig. more...

Organism:

Mus musculus; Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL22475 GPL21103

8 Samples

Download data: MTX, TSV

(Submitter supplied) 47,845 probe-sets were screened, This study compared expression profiles of mesenchymal stem cells derived from the two breed DL and AS and then analyzed the expression profile genes between two different sorts of synovial membrane in each breed.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16569

72 Samples

Download data: CEL

(Submitter supplied) Porcine deltacoronavirus (PDCoV) was a newly emerging and special delta coronavirus, which can infect mammals such as pigs, cattle and humans, as well as chickens and birds. RNA contains not only virus genetic coding information, but also structural information that regulating virus proliferation. In this study, the whole genome structure of PDCoV virion was detected by a virion RNA in situ conformation sequencing technology, named vRIC-seq. more...

Organism:

Sus scrofa

Type:

Other

Platform:

GPL22918

2 Samples

Download data: CT, HIC

(Submitter supplied) Sertoli cells (SCs), the only somatic constituent of the testicular seminiferous epithelium, are vital to spermatogenesis. We previously found that vitamin C (ascorbic acid, AA) can reprogram the transcriptome, and promote the proliferation and reproductive function of pig immature SCs (iSCs). However, the global change of microRNAs (miRNA) expression and its effect on pig iSCs as induced by vitamin C treatment is still unknown. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: TXT

(Submitter supplied) Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They can also contribute to the improved development of weaned piglets. However, their indigestible polysaccharides of the cell wall pose a challenge. This can be addressed through carbohydrase supplementation, such as the recombinant ulvan lyase. The objective of our study was to assess the muscle metabolism of weaned piglets fed with 7% U. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

17 Samples

Download data: CSV

(Submitter supplied) After observing the surgical wounds on the 28th postoperative day, the experimental animals were put to death. Vascular tissues from the surgical site were taken and subjected to gene microarray analysis. This was used to examine the effect of TAG repair in the experimental group and Exoseal repair in the control group.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL16571

6 Samples

Download data: TXT

(Submitter supplied) Pig-to-human xenotransplantation is rapidly approaching the clinical arena; however, it is unclear which immunomodulatory regimens will effectively control human immune responses to pig xenografts. Here, we transplant a gene-edited pig kidney into a brain-dead human recipient on pharmacologic immunosuppression and study the human immune response to the xenograft using spatial transcriptomics and single-cell RNA sequencing. more...

Organism:

Homo sapiens; Sus scrofa

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL24676 GPL26351

5 Samples

Download data: CSV, JPG, JSON, MTX, PNG, TSV

(Submitter supplied) LIN28A, an RNA-binding protein, plays an important role in porcine induced pluripotent stem cells (piPSCs). However, the molecular mechanism of LIN28A maintaining pluripotency in piPSCs remains unclear. The aim of this study was to explore the functions of LIN28A in piPSCs. Here we explored the functions of LIN28A in piPSCs by overexpressing and knocking down LIN28A. We performed total RNA sequencing (RNA-seq) on piPSCs and the expression levels of relevant-genes were detected by qRT-PCR, western blot and immunofluorescence staining. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26285

4 Samples

Download data: XLS

(Submitter supplied) We performed 10× Genomics single-cell RNA sequencing on a mixed population of CD3+ PBMCs isolated from three healthy Large White pigs. Considering that cell resolution of scRNA-seq analysis can be improved based on large datasets, we also downloaded 7 pbulic PBMC scRNA-seq datasets using the same technique (10× Genomics). After rigorous quality control and the removal of batch effects, a total of 14,595 transcriptomes were obtained from 34,220 cells in 8 PBMC samples for subsequent analyses. more...

Organism:

Sus scrofa domesticus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31437

1 Sample

Download data: MTX, TSV

(Submitter supplied) We found piRNAs with different lengths represented the predominant small RNA species in oocytes from the 12 explored species, except mouse. We found endo-siRNAs resulted from the truncated Dicer isoform were mouse-specific, and os-piRNAs associating with PIWIL3 in human oocytes are widespread in mammals and are typically with low levels of the 2’-3’-O-methylation. The sequences of many highly expressed piRNA clusters are fast-evolving compared with their syntenic genomic locations, and the TE families distributing in the conserved piRNA clusters are various between species.

Organism:

Macaca fascicularis; Oryctolagus cuniculus; Cricetulus griseus; Mesocricetus auratus; Mus musculus; Cavia porcellus; Danio rerio; Homo sapiens; Canis lupus familiaris; Sus scrofa domesticus; Capra hircus; Rattus norvegicus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

12 related Platforms

138 Samples

Download data

(Submitter supplied) Ex vivo lung perfusion (EVLP) has extended the number of transplantable lungs by reconditioning marginal organs. However EVLP is performed at 37°C without homeostatic regulation leading to metabolic wastes accumulation in the perfusate, and as a corrective measure, the costly perfusate is repeatedly replaced during the standard of care procedure. As an interesting alternative, a hemodialyzer could be placed on the EVLP circuit, that was previously shown to rebalance the perfusate composition, maintain lung function and viability without appearing to impact the global gene expression in the lung. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

27 Samples

Download data: TXT

(Submitter supplied) Heat stress (HS) could damage animal reproduction, while the mechanisms are still required to be better understood. Here, we showed that incubation of porcine immature Sertoli cells (iSCs) at 43 °C for 30 minutes followed 36h recovery under normal culture could inhibit the proliferation, promote apoptosis and increase the lactate production. Then, we profiled mRNA expression by RNA sequencing on Control (before HS), HS0.5 (0h after HS) and HS0.5-R36 (36h recovery after HS) groups of iSCs. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

9 Samples

Download data: XLS

(Submitter supplied) We measued IgG autoantibodies associated with Connective Tissue Diseases (CTDs) and Anti-Cytokine Antibodies (ACA) in SLE and healthy controls who received the BNT162b2 vaccine.

Organism:

Triticum aestivum; Capra hircus; Escherichia coli; Spodoptera frugiperda; Bos taurus; Homo sapiens; Sus scrofa

Type:

Protein profiling by protein array

Platform:

GPL34257

102 Samples

Download data: TXT, XLSX