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| Status |
Public on May 06, 2024 |
| Title |
N6-Methyladenosine RNA Modification Regulates the Differential Muscle Development in Large White and Ningxiang Pigs |
| Organism |
Sus scrofa |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
N6-methyladenosine (m6A) is the most common modification in eukaryotic RNAs. Accumulating evidence has shown that m6A methylation plays a vital role in various biological processes. However, research on the role of m6A modifications in regulation of porcine muscle growth is lacking. In this study, we identified differentially expressed genes in the early stages of muscle development between Large White (LW) and NingXiang (NX) pigs and further reported m6A methylation patterns via MeRIP-seq. We found that m6A modification regulates muscle cell development, myofibrils, cell cycle, and phosphatase regulator activity during the neonatal phase of muscle development. Interestingly, differentially expressed genes in LW and NX pigs were mainly enriched in pathways involved in protein synthesis. Furthermore, we performed a conjoint analysis of MeRIP-seq and RNA-seq data, and identified 27 differentially expressed and m6A-modified genes.
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| Overall design |
To gain insight into the identity of m6A-modified mRNAs involved in muscle development in LW and NX pigs, we performed m6A RNA immunoprecipitation followed by sequencing (meRIP-seq) of the longissimus dorsi of 5-day-old LW and NX pigs
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| Contributor(s) |
Gu H |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Apr 29, 2024 |
| Last update date |
May 06, 2024 |
| Contact name |
gu hao |
| E-mail(s) |
guhao@hbaas.com
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| Organization name |
Hubei Academy of Agricultural Sciences
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| Street address |
29 Nanhu Avenue, Shizishan Street
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| City |
wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
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| Platforms (1) |
| GPL26351 |
Illumina NovaSeq 6000 (Sus scrofa) |
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| Samples (12)
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| GSM8241013 |
NingXIANG pig muscle, replicate1, 5-day-old, MeRIP-seq |
| GSM8241014 |
NingXIANG pig muscle, replicate2, 5-day-old, MeRIP-seq |
| GSM8241015 |
NingXIANG pig muscle, replicate3, 5-day-old, MeRIP-seq |
| GSM8241016 |
LargeWhite pig muscle, replicate1, 5-day-old, MeRIP-seq |
| GSM8241017 |
LargeWhite pig muscle, replicate2, 5-day-old, MeRIP-seq |
| GSM8241018 |
LargeWhite pig muscle, replicate3, 5-day-old, MeRIP-seq |
| GSM8241019 |
NingXIANG pig muscle, replicate1, 5-day-old, Input |
| GSM8241020 |
NingXIANG pig muscle, replicate2, 5-day-old, Input |
| GSM8241021 |
NingXIANG pig muscle, replicate3, 5-day-old, Input |
| GSM8241022 |
LargeWhite pig muscle, replicate1, 5-day-old, Input |
| GSM8241023 |
LargeWhite pig muscle, replicate2, 5-day-old, Input |
| GSM8241024 |
LargeWhite pig muscle, replicate3, 5-day-old, Input |
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| Relations |
| BioProject |
PRJNA1105924 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266121_LW_1_Peak_Anno_knownGene.txt.gz |
150.9 Kb |
(ftp)(http) |
TXT |
| GSE266121_LW_2_Peak_Anno_knownGene.txt.gz |
141.1 Kb |
(ftp)(http) |
TXT |
| GSE266121_LW_3_Peak_Anno_knownGene.txt.gz |
161.3 Kb |
(ftp)(http) |
TXT |
| GSE266121_NX_1_Peak_Anno_knownGene.txt.gz |
129.9 Kb |
(ftp)(http) |
TXT |
| GSE266121_NX_2_Peak_Anno_knownGene.txt.gz |
135.3 Kb |
(ftp)(http) |
TXT |
| GSE266121_NX_3_Peak_Anno_knownGene.txt.gz |
142.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
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