|
| Status |
Public on Mar 06, 2024 |
| Title |
Divergent composition and transposon-silencing activity of small RNAs in mammalian oocytes |
| Organisms |
Danio rerio; Macaca fascicularis; Homo sapiens; Canis lupus familiaris; Sus scrofa domesticus; Capra hircus; Oryctolagus cuniculus; Cricetulus griseus; Mesocricetus auratus; Mus musculus; Rattus norvegicus; Cavia porcellus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Other
|
| Summary |
We found piRNAs with different lengths represented the predominant small RNA species in oocytes from the 12 explored species, except mouse. We found endo-siRNAs resulted from the truncated Dicer isoform were mouse-specific, and os-piRNAs associating with PIWIL3 in human oocytes are widespread in mammals and are typically with low levels of the 2’-3’-O-methylation. The sequences of many highly expressed piRNA clusters are fast-evolving compared with their syntenic genomic locations, and the TE families distributing in the conserved piRNA clusters are various between species.
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| |
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| Overall design |
Profiling small RNAs and mRNA in oocytes from 12 animals.
|
| Web link |
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-024-03214-w
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| |
|
| Contributor(s) |
Liu W, Hou L, Wu L |
| Citation(s) |
38532500 |
| |
| Submission date |
Apr 08, 2022 |
| Last update date |
Mar 27, 2024 |
| Contact name |
Wei Liu |
| E-mail(s) |
liuwei2018@sibcb.ac.cn
|
| Organization name |
Chinese Academy of Sciences
|
| Department |
Institute of Biochemistry and Cell Biology
|
| Street address |
320 Yueyang Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
| |
|
| Platforms (12)
|
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL24995 |
Illumina NovaSeq 6000 (Danio rerio) |
| GPL25760 |
Illumina NovaSeq 6000 (Canis lupus familiaris) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
| GPL26786 |
Illumina NovaSeq 6000 (Oryctolagus cuniculus) |
| GPL27106 |
Illumina NovaSeq 6000 (Capra hircus) |
| GPL27425 |
Illumina NovaSeq 6000 (Cricetulus griseus) |
| GPL28212 |
Illumina NovaSeq 6000 (Macaca fascicularis) |
| GPL28437 |
Illumina NovaSeq 6000 (Cavia porcellus) |
| GPL28997 |
Illumina NovaSeq 6000 (Mesocricetus auratus) |
| GPL29562 |
Illumina NovaSeq 6000 (Sus scrofa domesticus) |
|
| Samples (138)
|
|
| Relations |
| BioProject |
PRJNA824784 |
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