Similar studies for GEO DataSets (Select 2064) - GEO DataSets

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Items: 18

Select item 20641.

MicroRNA miR156b and miR164b overexpression effect on floral apex

Analysis of floral apices of transgenics overexpressing miRNA miR156b or miR164b. Most plant miRNAs have perfect or near-perfect complementarity with their targets. However, targets with up to 5 mismatches exist. Results provide insight into the mechanism of target recognition by plant miRNA.

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array, count, 3 genotype/variation sets

Platform:: GPL198
Series:: GSE2079
6 Samples

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DataSet

Accession:: GDS2064

ID:: 2064

Select item 2000020812.

Overexpression of miR319a (SD Leaves)

(Submitter supplied) jaw-D plants grown in SD compared to columbia wt. Keywords: parallel sample

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array

Dataset:: GDS2066
Platform:: GPL198
4 Samples

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Series

Accession:: GSE2081

ID:: 200002081

Select item 2000020803.

Overexpression of miR164b and 172a (vegetative apex)

(Submitter supplied) RNA was isolated from vegetative apices of 7d-old short-day grown plants. Overexpression of miR164b and miR172a versus control. Keywords: parallel sample

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array

Dataset:: GDS2065
Platform:: GPL198
6 Samples

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Series

Accession:: GSE2080

ID:: 200002080

Select item 2000020794.

Overexpression of miR156b and miR164b (floral apex)

(Submitter supplied) RNA was harvested from floral apices of long-day grown plants. Overexpression of miR156b and 164b versus control. Keywords: parallel sample

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array

Dataset:: GDS2064
Platform:: GPL198
6 Samples

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Series

Accession:: GSE2079

ID:: 200002079

Select item 2000020785.

Overexpression of miR159a

(Submitter supplied) Flowers stage 15: Comparison of miR159a overexpressers to empty-vector control. Keywords: parallel sample

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array

Dataset:: GDS2063
Platform:: GPL198
6 Samples

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Series

Accession:: GSE2078

ID:: 200002078

Select item 20666.

MicroRNA miR319a overexpression effect on the leaf

Analysis of leaves from transgenics overexpressing microRNA miR319a. Most plant miRNAs have perfect or near-perfect complementarity with their targets. However, targets with up to five mismatches exist. Results provide insight into the mechanistic basis of target recognition by plant miRNA.

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array, count, 2 genotype/variation sets

Platform:: GPL198
Series:: GSE2081
4 Samples

Download data

DataSet

Accession:: GDS2066

ID:: 2066

Select item 20657.

MicroRNA miR164b and miR172a overexpression effect on vegetative apex

Analysis of vegetative apices of transgenics overexpressing miRNA miR164b or miR172a. Most plant miRNAs have perfect or near-perfect complementarity with their targets. However, targets with up to 5 mismatches exist. Results provide insight into the mechanism of target recognition by plant miRNA.

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array, count, 3 genotype/variation sets

Platform:: GPL198
Series:: GSE2080
6 Samples

Download data

DataSet

Accession:: GDS2065

ID:: 2065

Select item 20638.

MicroRNA miR159a overexpression effect on flower

Analysis of stage 15 flowers from transgenics overexpressing miRNA miR159a. Most plant miRNAs have perfect or near-perfect complementarity with their targets. However, targets with up to five mismatches exist. Results provide insight into the mechanistic basis of target recognition by plant miRNA.

Organism:: Arabidopsis thaliana

Type:: Expression profiling by array, count, 2 genotype/variation sets

Platform:: GPL198
Series:: GSE2078
6 Samples

Download data

DataSet

Accession:: GDS2063

ID:: 2063

Select item 2000030089.

Small RNA identification in Arabidopsis thaliana using modified MPSS

(Submitter supplied) Small RNAs play important regulatory roles in most eukaryotes but only a small proportion of these molecules have been identified. We sequenced more than two million small RNAs from seedlings and the inflorescence of the model plant Arabidopsis thaliana. Known and new miRNAs were among the most abundant of the non-redundant set of more than 75,000 sequences, whereas more than half represented lower abundance small-interfering RNAs (siRNAs) that match repetitive sequences, intergenic regions, and genes. more...

Organism:: Arabidopsis thaliana

Type:: Expression profiling by MPSS

Platform:: GPL2675
4 Samples

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Series

Accession:: GSE3008

ID:: 200003008

PubMed Similar studies

Select item 20000944010.

Expression data from human embryonic stem cell differentiation

(Submitter supplied) MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3¡¦ untranslated region (3¡¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL570
8 Samples

Download data: CEL

Series

Accession:: GSE9440

ID:: 200009440

Select item 20001739811.

Transcriptome-wide identification of small RNA targets in rice

(Submitter supplied) MicroRNA (miRNA)-guided target RNA expression is vital for a wide variety of biological processes in eukaryotes. The integration of miRNAs in diverse biological networks relies upon the confirmation of their RNA targets. Most miRNA targets in Arabidopsis are validated, but those in rice are yet to be characterized. To identify transcriptome-wide small RNA targets in rice, we generated 20-nt small cDNA library and obtained nearly 40 million reads. more...

Organism:: Oryza sativa

Type:: Expression profiling by high throughput sequencing

Platform:: GPL9147
1 Sample

Download data: TXT

Series

Accession:: GSE17398

ID:: 200017398

PubMed Similar studies SRA Run Selector

Select item 20001569912.

Expression data in Arabidopsis ago1-25 and hyl1-2 mutants.

(Submitter supplied) We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome in Arabidopsis ago1-25 and hyl1-2 mutants.

Organism:: Arabidopsis thaliana

Type:: Expression profiling by genome tiling array

Platforms:: GPL10977 GPL10978
18 Samples

Download data: CEL, TXT

Series

Accession:: GSE15699

ID:: 200015699

PubMed Similar studies

Select item 20003968713.

Nitrogen-efficient rice over-expressing alanine aminotransferase

(Submitter supplied) Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa.

Organism:: Oryza sativa

Type:: Expression profiling by array

Platform:: GPL2025
32 Samples

Download data: CEL

Series

Accession:: GSE39687

ID:: 200039687

Select item 20002074814.

DNA Methylation Mediated by a MicroRNA Pathway

(Submitter supplied) In plants, the known microRNAs (miRNAs) are produced as ~21 nucleotide (nt) duplexes from their precursors by Dicer like 1 (DCL1). They are incorporated into Argonaute 1 (AGO1) protein to regulate target gene expression primarily through mRNA cleavage. We report here the discovery of a new class of miRNAs in the model monocot rice (Oryza sativa). These are 24 nt in length and require another member of the Dicer family, DCL3, for their biogenesis. more...

Organism:: Oryza sativa

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL9147
7 Samples

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Series

Accession:: GSE20748

ID:: 200020748

PubMed Similar studies SRA Run Selector

Select item 20006221715.

Small RNA Solexa sequencing of Chinese sacred lotus (Nelumbo nucifera Gaertn.)

(Submitter supplied) The total RNA were extracted from pooled tissues of leaves and flowers from several plants of sacred lotus using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Then small RNAs ranging in 18–30 nucleotides were size fractionated electrophoretically, isolated from the gel, ligated with the 5′ and 3′ RNA adapters. The ligated product was reverse transcribed and subsequently amplified using 10–12 PCR cycles. more...

Organism:: Nelumbo nucifera

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL19280
2 Samples

Download data: TXT

Series

Accession:: GSE62217

ID:: 200062217

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20004888616.

Small RNAs from Nicotiana benthamiana leaves

(Submitter supplied) Small RNA expression from Nicotiana benthamiana leaves was profiled with the primary goal of ascertaining microRNA isoform diversity for known, conserved families. A secondary goal was to provide a baseline small RNA expression atlas for this species and tissue.

Organism:: Nicotiana benthamiana

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL17448
4 Samples

Download data: TXT

Series

Accession:: GSE48886

ID:: 200048886

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20005024917.

Systematic design and functional analysis of artificial microRNAs as an approach for multi-gene targeting

(Submitter supplied) Unlike short interfering RNAs (siRNAs), which are commonly designed to repress a single messenger RNA (mRNA) target through perfect base pairing, microRNAs (miRNAs) are endogenous small RNAs that have evolved to concurrently repress multiple mRNA targets through imperfect complementarity. MicroRNA target recognition is primarily determined by pairing of the miRNA seed sequence (nucleotides 2–8) to complementary match sites in each mRNA target. more...

Organism:: Homo sapiens

Type:: Expression profiling by array

Platform:: GPL10558
15 Samples

Download data: TXT

Series

Accession:: GSE50249

ID:: 200050249

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 20014757618.

Tissue-dependent regulation of miRNA activity

(Submitter supplied) miRNAs play important roles in every aspect of plant development. Small RNA regulation confers sensitivity and robustness onto gene regulatory networks, and the morphogen-like readout of small RNA mobility gradients yields sharply delineated domains of target gene expression. However, how the spatiotemporal patterns of miRNA activity are attained is less well understood. We used the high-resolution maize shoot apex transcriptome atlas (Knauer et al., 2019) together with data from small RNA-Seq, PARE analysis, small RNA in-situ hybridization, and RNAseq of weak dcl1 mutant tissues to demonstrate that miRNA accumulation and function during development is regulated in a tiissue dependent manner at the transcriptional and post-transcriptional level.