GEO DataSets

(Submitter supplied) The goals of this study are to compare NGS-derived whole transcriptome profiles (RNA-seq) of H5N1 infected A549 cells overexpressing either negative control mimic or miR-324-5p mimic

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

3 Samples

Download data: TXT

(Submitter supplied) Mammals have highly evolved receptors for sensing invading viral pathogens. RLRs comprise of one such family of receptors involved in sensing RNA viruses. The antiviral signaling cascade is tightly regulatedd by various post transcriptional modifications however the post transcriptional regulation by microRNAs is not well understood. We performed miRNA profiling to identify miRNAs induced by virus infections with potential role in regulating RLR signaling cascade.

Organism:

synthetic construct; Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL19117

4 Samples

Download data: CEL

(Submitter supplied) To study miRNA expression profiles during highly pathogenic avian influenza virus infection, we conducted global miRNA expression profiling in human lung epithelial cells (A549) with or without H5N1 IAV infection. .

Organism:

Homo sapiens; Human alphaherpesvirus 1; Human betaherpesvirus 5; Betapolyomavirus macacae; Lymphocryptovirus; Human immunodeficiency virus 1

Type:

Non-coding RNA profiling by array

Platform:

GPL19221

6 Samples

Download data: TXT

(Submitter supplied) Influenza A virus (IAV) is a human respiratory pathogen that causes yearly global epidemics, and sporadic pandemics due to human adaptation of pathogenic strains. Efficient replication of IAV in different species is, in part, dictated by its ability to exploit the genetic environment of the host cell. To investigate IAV tropism in human cells, we evaluated the replication of IAV strains in a diverse subset of epithelial cell lines. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

8 Samples

Download data: TXT

(Submitter supplied) To further understand the roles of miRNA during influenza A virus infection, we performed miRNA profiling in human alveolar adenocarcinoma cell lines, A549 cells, infected with influenza A virus A/Beijing/501/2009(H1N1) and A/goose/Jilin/hb/2003(H5N1).

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL16770

18 Samples

Download data: TXT

(Submitter supplied) To investigate whether influenza A virus (IAV) infection alters cellular miRNA profiles, A549 cells were either mock-infected or infected with two different subtypes of IAV, A/Beijing/501/2009 (H1N1; MOI=5) and A/Vietnam/1194/2004 (H5N1; MOI=2), for 24 or 48 h. Total RNA was purified from cell samples, and microarray analysis of miRNAs was performed. The expression of selected miRNAs was further confirmed by quantitative real-time PCR.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL8179

6 Samples

Download data: IDAT, TXT

(Submitter supplied) MicroRNA-203 was up-regulated markedly upon H5N1 virus infection. To identify the potential target genes of miR-203, we constructed a miR-203 knockout A549 cell line. Then wild-type and miR-203 knockout A549 cells were mock-infected or infected with H5N1 virus for 48h. The Agilent Whole Human Genome Oligo Microarray was performed to analyze the mRNA expression profiling. Meanwhile, the online tool TargetScanHuman (http://www.targetscan.org/vert_71/) was used to predict biological targets of miR-203. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

18 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Recently, a novel protein in the influenza virus segment 3 has been identified, namely PA-X. This small protein has been reported to play a role in modulating host response of the 1918 H1N1 pandemic virus-infected mice. However, poteinal role of this protein in the pathogenicity and regulating host response of the highly pathogenic H5N1 virus in a chicken animal model is completely unknown. We used microarray analysis to evaluate the global transcriptional response in the lungs of the chickens infected with the parental strain (CK10) and PA-X deficiency mutant strain (CK-PAX3).

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL3213

9 Samples

Download data: CEL

(Submitter supplied) Highly pathogenic avian influenza virus (HPAIV) is a permanent threat due to its capacity to cross species barriers and generate severe infections and high mortality in humans. Recent findings have highlighted the potential role of PB1-F2, a small accessory influenza protein, in the pathogenesis process mediated by HPAIV in mammals. In this study, using a recombinant H5N1 HPAIV (wt) and its PB1-F2-deleted mutant (ΔF2), we studied the effects of PB1-F2 in a chicken model. more...

Organism:

Gallus gallus

Type:

Expression profiling by array

Platform:

GPL13790

10 Samples

Download data: TXT

(Submitter supplied) To evaluate the change of miRNA expression profile in DF-1 cells in respond to Infectious bursal disease virus (IBDV) infection, Deep sequencing was performed by LC Sciences (Hangzhou, China) on DF-1 cells infected with mock or IBDV Lx strain at an MOI of 1 for 24 h.

Organism:

Gallus gallus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19005

2 Samples

Download data: XLS

(Submitter supplied) An H5N1 virus-encoded microRNA directly targets mammalian poly(rC) binding protein 2 and is a major contributor to H5N1-associated ‘cytokine storm’ and mortality.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL10999

1 Sample

Download data: TXT

(Submitter supplied) Although accumulating evidence has shown that long non-coding RNAs (lncRNAs) are involved in multiple biological processes, considerably less is known regarding their functions in influenza A virus (IAV) replication. Here, lncRNA expression profiles were determined by RNA sequencing in three pairs of influenza virus A/Puerto Rico/8/34 (H1N1)-infected or uninfected A549 cells.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21290

6 Samples

Download data: FA, GTF, TXT

(Submitter supplied) Influenza A viruses (IAVs), particularly H1N1, H5N1 and H7N9, pose a substantial threat to public health worldwide. Here, we report that MIR2911, a honeysuckle (HS)-encoded atypical microRNA, directly targets IAVs with a broad spectrum. MIR2911 is highly stable in HS decoction, and continuous drinking or gavage feeding of HS decoction leads to a significant elevation of the MIR2911 level in mouse peripheral blood and lung. more...

Organism:

Lonicera japonica

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18317

2 Samples

Download data: TXT

(Submitter supplied) Occasional transmission of highly pathogenic avian H5N1 influenza viruses to humans causes severe pneumonia with high mortality. To better understand the mechanisms via which H5N1 viruses induce severe disease in humans, we infected cynomolgus macaques with six different H5N1 strains isolated from human patients and compared their pathogenicity and the global host responses to the virus infection. Although all H5N1 viruses replicated in the respiratory tract, there was substantial heterogeneity in their replicative ability and in the disease severity induced, which ranged from asymptomatic to fatal. more...

Organism:

Macaca mulatta

Type:

Expression profiling by array

Platform:

GPL14569

45 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL9115

18 Samples

Download data: TXT

(Submitter supplied) For the small RNA portion in the whole transcriptome, a total of 105,842,096 filtered high-quality sequencing reads, representing 2,854,995 unique miRNA species, were generated for miRNAs for H1N1- or H5N1- infected macrophage cell populations at 1-, 3-, and 6-hr post-infection. A total of 361 mature miRNAs and 113 mature star miRNAs were identified from the samples. We calculated the miRNA expression fold change in influenza A viruses infected cells compared to mock-infected control cells andassessed the significance of the differential expression for each miRNA using Z-statistics. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9115

9 Samples

Download data: XLS

(Submitter supplied) We generated an average of 868 million nucleotides and 30 folds coverage per sample. More than 76% of the sequencing reads were mapped to the human genome, and 45.5% of the annotated reference genes were mapped by at least 10 reads. Differential expression quantification showed that H5N1 virus elicits greater changes in host gene expression than the H1N1 virus at nearly all three time points with 1198,273,and 620 up-regulated in H5N1, and 426,285, and 523 in H1N1 compared with mock control (fold-change >= 2.0, and p-value <= 0.05). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9115

9 Samples

Download data: TXT, XLS

(Submitter supplied) Macrophages were infected with low (PR8) and high pathogenic influenza viruses (FPV and H5N1). To our surprise a genome-wide comparative systems biology approach revealed that in contrast PR8 infections with HPAIV H5N1 and FPV result in a reduced immune response of human macrophages contradicting a primary role of this cell type for the cytokine storm. Our data point to a viral strategy of HPAIV to bypass a major amplifier of the initial local inflammatory response thereby hampering antiviral effector mechanisms and facilitating virus spreading and systemic disease.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL571

12 Samples

Download data: CEL, CHP