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| Status |
Public on May 09, 2018 |
| Title |
Microarray analysis of microRNAs expression in A549 cells during influenza A virus infection |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To investigate whether influenza A virus (IAV) infection alters cellular miRNA profiles, A549 cells were either mock-infected or infected with two different subtypes of IAV, A/Beijing/501/2009 (H1N1; MOI=5) and A/Vietnam/1194/2004 (H5N1; MOI=2), for 24 or 48 h. Total RNA was purified from cell samples, and microarray analysis of miRNAs was performed. The expression of selected miRNAs was further confirmed by quantitative real-time PCR.
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| Overall design |
A549 cells were mock-infected or infected with two different subtypes of IAV, A/Beijing/501/2009 (H1N1; MOI=5) and A/Vietnam/1194/2004 (H5N1; MOI=2), for 24 or 48 h. Then total RNA was purified from cell samples to measure the microRNAs expression.
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| Contributor(s) |
Zhang S, Hu Y |
| Citation(s) |
29717211, 37631988 |
| |
| Submission date |
Nov 20, 2017 |
| Last update date |
Sep 15, 2023 |
| Contact name |
Sen Zhang |
| E-mail(s) |
zhangsen2260@163.com
|
| Organization name |
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology
|
| Street address |
East Street of Fengtai
|
| City |
Beijing |
| ZIP/Postal code |
100071 |
| Country |
China |
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| Platforms (1) |
| GPL8179 |
Illumina Human v2 MicroRNA expression beadchip |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA419225 |
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