GEO DataSets

(Submitter supplied) T-47D breast cancer cells were stably transfected with a tet-off inducible construct encoding estrogen receptor beta. Microarray experiments were carried out at multiple time points 1-30 hours following treatment with estradiol and under induction and non-induction conditions. Keywords: Inducible expression of ERbeta

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4840

20 Samples

Download data: GPR

(Submitter supplied) Purpose: Endocrine therapies, such as tamoxifen are commonly given to most patients with estrogen receptor (ER) alpha-positive breast carcinoma but are not indicated for persons with ERalpha-negative cancer. The factors responsible for response to tamoxifen in 5-10% of patients with ERalpha-negative tumors are not clear. The aim of the present study was to elucidate the biology and role of the second ER, ERbeta, in patients treated with adjuvant tamoxifen. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2827

Platform:

GPL3883

88 Samples

Download data: GPR

Analysis of estrogen receptor (ER) alpha negative ERbeta-positive breast cancer tumors from patients treated with tamoxifen for 2 years. Unlike ERalpha-negative ERbeta-negative breast cancers, ERalpha-negative ERbeta-positive cancers respond favorably to tamoxifen treatment.

Organism:

Homo sapiens

Type:

Expression profiling by array, log2 ratio, 6 specimen sets

Platform:

GPL3883

Series:

GSE6577

88 Samples

Download data: GPR

(Submitter supplied) The prognosis of a patient with Estrogen Receptor (ER) and/or Progesterone Receptor (PR)-positive breast cancer is highly variable. Therefore, we developed a gene-expression based outcome predictor for ER+ and/or PR+ (i.e. Luminal) breast cancer patients using biological properties of the tumors. First, we identified estrogen-regulated genes using the ER+ MCF-7 breast cancer cell line treated with estrogen. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

174 Samples

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(Submitter supplied) The beneficial effect of the selective estrogen receptor modulator (SERM) tamoxifen in the treatment and prevention of breast cancer is assumed to be through its ability to antagonize the stimulatory actions of estrogen, although tamoxifen can also have some estrogen-like agonist effects. Here we report that in addition to these mixed agonist/antagonist actions, tamoxifen can also selectively regulate a unique set of more than 60 genes in estrogen receptor alpha (ERa)-positive MCF-7 human breast cancer cells which are minimally regulated by estradiol or raloxifene. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2367

Platform:

GPL96

17 Samples

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Analysis of estrogen receptor alpha (ERalpha)-positive MCF-7 breast cancer (BC) cells infected with adenovirus-ERbeta and treated with tamoxifen (Tam). Tam is a selective ER modulator used in BC prevention and treatment. Results provide insight into Tam activity in the presence of both ERs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 agent, 2 infection sets

Platform:

GPL96

Series:

GSE4025

17 Samples

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(Submitter supplied) Primary human breast tumors were obtained from the National Cancer Centre of Singapore (NCC) Tissue Repository, after appropriate approvals from the NCC Repository and Ethics Committees. Profiled samples contained at least 50% tumor content. Keywords = Gene expression Keywords = Breast Cancer Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

96 Samples

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(Submitter supplied) Two subtypes of the estrogen receptor, ERalpha and ERbeta, mediate the actions of estrogens, and the majority of human breast tumors contain both ERalpha and ERbeta. To examine the possible interactions and modulatory effects of ERbeta on ERalpha activity, we have used adenoviral gene delivery to produce human breast cancer (MCF-7) cells expressing ERbeta, along with their endogenous ERalpha. We have examined the effects of ERβ expression on genome-wide gene expression by Affymetrix GeneChip microarrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2770

Platform:

GPL96

12 Samples

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Analysis of estrogen receptor (ER) alpha positive MCF-7 breast cancer cells following introduction of ERbeta and treatment with estrogen. The majority of breast cancers express both ERalpha and ERbeta. Results provide insight into the comodulatory effects of these two ERs.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 infection sets

Platform:

GPL96

Series:

GSE4006

12 Samples

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(Submitter supplied) To identify microRNAs impacting estrogen receptor ERα expression in breast cancer, we have screened ER-positive breast cancer cells with a library of pre-miRs, and systematically monitored the ERα expression by protein lysate microarrays. There was a significant enrichment of the in silico predicted ERα targeting microRNAs among the hits. The most potent pre-miRs miR-18a/b, miR-193b, miR-206, and miR-302c, were confirmed to directly target ERα and to repress estrogen-responsive genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

11 Samples

Download data: TXT

(Submitter supplied) We were interested in determining what genes might be controlled by TFAP2C and/or TFAP2A, either directly or indirectly through regulation of ER-alpha and potentially other signaling pathways. We performed an microarray analysis in MCF7 cells with elimination of either TFAP2C or TFAP2A. The patterns of gene expression with alteration of TFAP2 activity were compared to changes in expression induced by estrogen exposure. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP

(Submitter supplied) BACKGROUND: The AP2 transcription factor family is a set of developmentally regulated, retinoic acid (RA) inducible genes, which regulate expression of estrogen receptor-alpha (ERalpha) in breast carcinoma. We hypothesized that AP2 factors regulate a set of genes characteristic of the hormone responsive breast cancer phenotype. To better understand the role of AP2 factors in hormone responsive breast cancer, we sought to identify AP2-target genes in breast epithelial cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL2670 GPL3147

4 Samples

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(Submitter supplied) Obesity is thought to contribute to worse disease outcome in breast cancer as a result of increased levels of adipocyte-secreted endocrine factors, insulin, and insulin-like growth factors (IGFs) that accelerate tumor cell proliferation and impair treatment response. We examined the effects of patient obesity on primary breast tumor gene expression, by profiling transcription of a set of tumors for which the patients’ body mass index (BMI) was ascertained. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL96

103 Samples

Download data: CEL

(Submitter supplied) We performed chromatin immunoprecipitation (ChIP) with overnight with antibodies against the C-terminus (HC-20, from Santa Cruz Biotechnology, Europe) or the N-terminus (anti-Estrogen Receptor 18-32, from SigmaAldrich, Italy) of human ER-alpha with or without estrogen treatment for 45 minutes on TAP-ER-beta cells, and the immunoprecipitated DNA was sequenced with the Illumina/Solexa Genome Analyzer. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9115

8 Samples

Download data: TXT

(Submitter supplied) Analysis of 104 breast cancer biopsies (removed prior to any treatment with tamoxifen or chemotherapeutic agents) from patients aged between 31 years and 89 years at the time of diagnosis (mean age = 58 years). Twenty were less than 50 years and seventy-seven women were 50 years, or older, at diagnosis. The size of the tumours ranged between 0.6 cm and 8.0 cm (mean = 2.79 cm). Eighteen tumours were T1 (<2 cm) in maximal dimension; 83 were T2 (2–5 cm) and 3 tumours were T3 (>5 cm). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

121 Samples

Download data: CEL

(Submitter supplied) In order to elucidate the mechanism of ERβ in the colon, we validated ERβ antibodies, opti-mized chromatin-immunoprecipitation (ChIP), and performed ChIP-Seq of CRC engineered cell lines re-expressing ERβ.We present for the first time, the cistrome of nuclear receptor ERβ in different CRC cell lines.

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21697

11 Samples

Download data: BEDGRAPH

(Submitter supplied) Changes in the expression of 8400 genes were monitored by cDNA microarray analysis during the first 32 h of human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose of 17beta-estradiol, a timing which corresponds to completion of a full mitotic cycle in hormone-stimulated cells. Hierarchical clustering of 344 genes whose expression either increases or decreases significantly in response to estrogen reveals that the gene expression program activated by the hormone in these cells shows 8 main patterns of gene activation/inhibition. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL970

25 Samples

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(Submitter supplied) Plasma cell leukemia (PCL) is a rare form of plasma cell dyscrasia that presents either as a progression of previously diagnosed multiple myeloma (MM), namely secondary PCL (sPCL), or as the initial manifestation of disease, namely primary PCL (pPCL). Although presenting signs and symptoms include those seen in MM, pPCL is characterized by several aspects that clearly define more aggressive course. To provide insights into the biology of pPCL, we have investigated the transcriptional profiles of a cohort of 21 newly-diagnosed, homogeneously treated pPCL patients included in a multicenter prospective clinical trial. All but one pPCL had one of the main IGH translocations, whose associated transcriptional signatures resembled those observed in MM. A 503-gene signature was identified that distinguished pPCL from MM, from which emerged 28 genes whose trend in expression levels was found associated with the progressive stages of plasma cell dyscrasia in a large dataset of cases from multiple institutions, including samples from normal donors throughout PCL. The transcriptional pattern of the pPCL series was then evaluated in association with outcome. Three genes were identified having expression levels correlated with response to the first-line treatment with lenalidomide/dexamethasone, whereas a 27-gene signature was identified associated with overall survival independently of molecular alterations, hematological parameters and renal function. Overall, our data contribute to a fine dissection of pPCL and may provide novel insights into the molecular definition of a subgroup of high-risk pPCL.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

76 Samples

Download data: CEL

(Submitter supplied) This experiment set contains the complete set of raw data for Whitfield et al. (2002)Mol.Biol.Cell. HeLa S3 cells were synchronized by three different methods (double thymidine block, thymidine-nocodazole block or mitotic shake-off)in five independent time courses. mRNA from the first and second double thymidine block experiments were converted to cDNA by standard methods using an oligo-dT/random hexamer mix and hybridized to 24K arrays; mRNA from the third double thymidine and thymidine-nocodazole block experiments were converted to cDNA using olido-dT alone and hybridized to 43K arrays; total RNA from the mitotic shake-off experiment was first amplified using a modified eberwine protocol, aRNA converted to cDNA by reverse transcription with random hexamer and hybridized to 43K arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

6 related Platforms

114 Samples

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