GEO DataSets

(Submitter supplied) Gene expression differences between MR-VL_FBO, a T. urticae strain highly resistant to fenbutatin oxide, and KOP8, a T. urticae strain susceptible to fenbutatin oxide, were determined.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over-expression of P450s and other detoxification genes in the same field population is reported.

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28937

12 Samples

Download data: TXT

(Submitter supplied) We performed expression quantitative trait locus (eQTL) mapping between two strains of Tetranychus urticae (the two-spotted spider mite), a generalist herbivore known for its rapid evolution of acaricide resistance. For parents, we used the inbred strain MR-VPi that is highly resistant to multiple acaricides in different classes, and the inbred strain ROS-ITi that is comparatively susceptible to many acaricides. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28937

506 Samples

Download data: TXT

(Submitter supplied) While performing mitochondrial isolations and recently developed tRNA-seq methods (AlkB treatment and YAMAT-Seq) in plant tissue, we inadvertently sequenced the mitochondrial tRNAs from a common plant pest, the acariform mite Tetranychus urticae, to a high enough coverage to detect all previously annotated T. urticae tRNA regions. The results not only confirm expression, CCA-tailing and post-transcriptional base modification of these highly divergent tRNAs, but also revealed paired sense and antisense expression of multiple T. more...

Organism:

Tetranychus urticae

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL28937

6 Samples

Download data: FASTA, TXT

(Submitter supplied) No major transcriptomic differences were observed between JPR-R1/2 and JPR when strains were not exposed to pyflubumide. Only three genes (tetur13g01730, tetur14g03160, and tetur14g01700) were consistently up-regulated in JPR-R1/2, but none coded for enzymes that are known to be associated with xenobiotic metabolism. As a next step, we used the original and susceptible JPO strain, the parent of JPR, as a reference to investigate transcriptomic changes. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

24 Samples

Download data: TXT

(Submitter supplied) Macrocylic lactones, milbemectin and abamectin, are compounds that are frequently used for the control of the two-spotted spider mite Tetranychus urticae. The development of abamectin resistance in this major pest has become an increasing problem worldwide, potentially compromising the use of milbemectin. In this study, gene expression profile differences between abamectin/milbemectin resistant T. urticae populations (ES1, IT2 and UK6) and a susceptible T. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL23384 GPL28237

20 Samples

Download data: FASTA, TXT

(Submitter supplied) In this study, we show that in a spider mite species, Tetranychus urticae, genetic changes do not drive experimental range expansions. We performed two experiments, each with two treatments, wherein mite populations were allowed to expand their habitat range. In a first experiment, we either replaced migrating females with females from the parental stock populations (Replacement From Stock) or left the migrants unmanipulated (Control). more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

11 Samples

Download data: TXT

(Submitter supplied) Synergists can counteract metabolic insecticide resistance by inhibiting detoxification enzymes or transporters. In this study we used Illumina RNA-sequencing to investigate genome-wide transcriptional responses in an acaricide resistant strain (JP-R) of the spider mite Tetranychus urticae upon exposure to synergists such as S,S,S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PBO) and cyclosporin A (CsA).

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23384

24 Samples

Download data: TXT

(Submitter supplied) Cyflumetofen is a novel acaricide on the international market with an unknown mode of action. Under laboratory conditions, we selected for high levels of cyflumetofen resistance in a susceptible mite strain and performed genome-wide gene-expression analysis. Differential expression between the resistant and susceptible strain was identified to study on the molecular level how mites develop resistance to this novel acaricide. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) Genome annotation of the chelicerate Tetranychus urticae revealed the absence of many canonical immunity genes. T. urticae either does not mount an immune response or it induces uncharacterized immune pathways. To disentangle these two hypotheses, we performed transcriptomic analysis of mites injected with bacteria vs mites injected with LB-buffer. Two types of bacteria were injected: E. coli and B. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

26 Samples

Download data: TXT

(Submitter supplied) Sex-specific differences in gene expression underlie differences in morphology, behavior, and reproduction. To date, little is known about sex-specific differences in gene expression in spider mites, even though males and females differ markedly in morphology and behavior. In this study, we describe the complement of sex-specific gene expression differences between males and females of the two-spotted spider mite (Tetranychus urticae), an important generalist herbivore that is a significant crop pest. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21813

8 Samples

Download data: TXT

(Submitter supplied) Proteins produced in the salivary glands of arthropod herbivores can function as effectors to modify plant defense responses. To obtain a set of candidate, salivary gland specific genes for the mite herbivore Tetranychus urticae, mites were dissected and the head region was recovered. Specifically, the proterosoma was dissected from 250 mites. This includes the salivary glands, and other nearby tissue (given their small size, exact micro-dissection of salivary glands was not possible). more...

Organism:

Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21813

4 Samples

Download data: TXT

(Submitter supplied) A genetically diverse strain (labelled as London) of the phytophagous mite Tetranychus urticae was transferred from its common host (bean) to other host plants (cotton, maize or soy). Three different host plant species were included in the experimental set-up: cotton (Gossypium spp.), maize (Zea mays cv. Ronaldinio) and soy (Glycine max cv. Merlin). Five generations after host-transfer, total RNA of all mite populations (London, London-SOY, London-MAIZE and London-COTTON) was collected and used in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) Microarray analysis revealed large-scale differential expression of genes coding for enzymes of detoxification families, secreted proteins with unknown functions and regulatory enzymes.

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

11 Samples

Download data: TXT

(Submitter supplied) In this study, we profiled the transcriptional changes in a polyphagous spider mite, Tetranychus urticae, after adaptation to spatial and tempospatial stress. We show heritable down-regulation of genes encoding for core enzymes involved in the citric acid and gluconeogenesis/glycolyse pathways (glucose 6-phosphatase among others). Additionally, we observe heritable transcriptional changes in amino acid pathways (methionine, tyrosine and phenylalanine) and in laterally acquired genes from bacteria (cobalamin-independent methionine synthase). more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

6 Samples

Download data: TXT

(Submitter supplied) Cyenopyrafen is a novel acaricide with a currently unknown mode of action. We selected for high levels of cyenopyrafen resistance in a susceptible spider mite strain and previously published patterns of cross-resistance to other well-described acaricides (Khalighi et al., 2014). In addition, we also included an independent cyenopyrafen-susceptible mite strain (referred to as Akita) in this study. We performed transcriptomic analysis to identify on the molecular level how T. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

8 Samples

Download data: TXT

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of differentially expressed genes (DEG) between the treated samples of mites.The goal of the study is to elucidate the invasion mechanism that results in the continuous expansion of Tetranychus urticae that has occurred in China. Methods:Transcriptome profiling by RNA sequencing is becoming an attractive method as it facilitates rapid generation, identification and quantification of large number of transcripts, even in the species where no prior genome sequence information is available. more...

Organism:

Tetranychus cinnabarinus; Tetranychus urticae

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21192 GPL21191

24 Samples

Download data: TXT

(Submitter supplied) In an experimental evolutionary set-up, we transferred a genetically diverse strain of the spider mite Tetranychus urticae from its common host bean to tomato where replicated populations were allowed to adapt. By sampling the transcriptomes of non-adapted and adaptes mites feeding on bean and tomato, we identified gene-expression changes in the spider mite affiliated with tomato adaptation. Transcriptional analysis revealed that both constitutive gene-expression levels as well as the transcriptional plasticity of genes were affected. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

12 Samples

Download data: TXT

(Submitter supplied) Nilaparvata lugens, the brown planthopper (BPH) sucks the rice phloem sap containing high sucrose to obtain carbon source. The comparative gene expression analyses were perfomed during feeding against starvation in order to determine sugar transporter and other feeding related gene expression.

Organism:

Tetranychus urticae; Nilaparvata lugens

Type:

Expression profiling by array

Platform:

GPL20083

6 Samples

Download data: TXT

(Submitter supplied) 250 adult T. urticae females from the London strain (grown on acyanogenic P. vulgaris cv. Prelude bean plants) were transferred to cyanogenic P. lunatus cv. 8078 bean plants. Thirty-five generations after the host transfer, total RNA was extracted from mites growing on both bean species (London and London-CYANO strain) and used in in a genome-wide gene expression microarray (Sureprint G3 microarray, Agilent) experiment to assess significantly differentially expressed genes (FC ≥ 2 and FDR-corrected p-value < 0.05) between mites grown on P. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT

(Submitter supplied) Investigating essential physiological processes in diapausing mites by analyzing genome wide gene expression changes using custom-built microarray. We investigated the molecular biology of facultative reproductive diapause in the chelicerate Tetranychus urticae (Acari: Tetranychidae) by analyzing genome-wide gene expression differences in diapausing and non diapausing T. urticae, using an Agilent custom-built two color gene expression microarray. more...

Organism:

Tetranychus urticae

Type:

Expression profiling by array

Platform:

GPL16890

4 Samples

Download data: TXT