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| Status |
Public on Oct 02, 2017 |
| Title |
The effect of insecticide synergist treatment on genome-wide gene expression in a polyphagous pest |
| Organism |
Tetranychus urticae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Synergists can counteract metabolic insecticide resistance by inhibiting detoxification enzymes or transporters. In this study we used Illumina RNA-sequencing to investigate genome-wide transcriptional responses in an acaricide resistant strain (JP-R) of the spider mite Tetranychus urticae upon exposure to synergists such as S,S,S-tributyl phosphorotrithioate (DEF), diethyl maleate (DEM), piperonyl butoxide (PBO) and cyclosporin A (CsA).
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| Overall design |
In total 24 RNA-seq samples (6 treatments, 4 biological replicates per treatment) from Tetranychus urticae, were generated. RNA was collected from adult T. urticae females 24h after being sprayed with a synergist compound (PBO, DEF, DEM or CsA) or with formulation only (FORM). Unsprayed mites served as an additional control (CON). For each sample, Illumina mRNA-seq libraries were constructed and strand-specific paired reads of 2x125 bp were generated. The obtained reads were used to quantify gene expression in each sample.
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| Contributor(s) |
Snoeck S, Greenhalgh R, Tirry L, Clark RM, Van Leeuwen T, Dermauw W |
| Citation(s) |
29044179 |
| |
| Submission date |
Apr 27, 2017 |
| Last update date |
May 24, 2019 |
| Contact name |
Wannes Dermauw |
| E-mail(s) |
wannes.dermauw@ugent.be
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| Phone |
003292646192
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| Organization name |
University Ghent
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| Department |
Crop Protection
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| Lab |
Agrozoology
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| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL23384 |
Illumina HiSeq 2500 (Tetranychus urticae) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA384641 |
| SRA |
SRP105368 |
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