GEO DataSets

(Submitter supplied) The glycopeptide vancomycin is a drug of choice for the treatment of severe non-gastrointestinal infections with members of Bacillus cereus sensu lato. Recently, sporadic detection of vancomycin resistant phenotypes emerged, mostly for agar diffusion testing. The food packaging isolate BC70 displayed a pseudo-resistant phenotype for vancomycin in both Etest and disk diffusion. In this work, we used RNA-Seq on the nanopore platform to study differentially expressed genes in BC70 cells, which were able to actively move into the inhibition zone during vancomycin susceptibility testing using Etest and therefore appeared to be resistant. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34081

4 Samples

Download data: CSV

(Submitter supplied) The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest.

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26265

6 Samples

Download data: GCT, GFF, TSV, TXT, XLSX

(Submitter supplied) Transcriptome analyses after the transfer of pCER270-spec megaplasmid in B. cereus F4810-72 (previously cured for its plasmid), B. thuringiensis HD73, and B. weihenstephanensis KBAB6 strains. We determined differentially expressed genes in pCER270+ strains in comparison with pCER270- strains.

Organism:

Bacillus cereus; Bacillus thuringiensis; Bacillus mycoides

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29834 GPL32378 GPL32936

36 Samples

Download data: TXT

(Submitter supplied) Bacillus cereus is the second leading cause of collective food poisoning in France. B. cereus is also associated with severe clinical infections leading to patient death in 10% of the cases. The emergence of B. cereus as a foodborne and opportunistic pathogen has intensified the need to distinguish strains of public health concern. In this work, by performing a screen on a large collection of B. cereus strains of varying pathogenic potential, we identified genetic determinants capable of discriminating B. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

45 Samples

Download data: CSV

(Submitter supplied) Bacterial response to nitric oxide (NO) is of major importance for bacterial survival, as NO stress is a main actor of the eukaryote immune response. Several pathogenic bacteria have developed gene regulation systems involved in detoxification and in repairing the damages caused by NO. However, bacterial mechanisms of NO resistance are poorly described for Gram positive bacteria and especially Bacillus cereus. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

21 Samples

Download data: CSV

(Submitter supplied) In the intestine, B. cereus comes in contact with the epithelial mucus layer. To investigate the overall response to that contact, total transcriptome analyses were performed using porcine gastric mucin (PGM), which is a commonly applied model for mucus interactions. Differential regulation of genes encoding enterotoxins and further virulence factors was of special interest.

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26265

6 Samples

Download data: TSV

(Submitter supplied) We investigated an improved method that combines competitive PCR and microarray techniques. This approach allowed us to quantify specific bacterial groups mounted on DNA chips with accuracy close to that of real-time PCR, despite a measurement at the end point of PCR, and also to estimate the bacterial DNA content in sample DNA.

Organism:

Treponema denticola; Cereibacter sphaeroides; Streptococcus gordonii; Streptococcus agalactiae; Enterococcus faecalis; Bifidobacterium adolescentis; Homo sapiens; Prevotella intermedia; Prevotella nigrescens; Neisseria meningitidis; Porphyromonas gingivalis; Fusobacterium nucleatum; Clostridium beijerinckii; Lactobacillus gasseri; Tannerella forsythia; Campylobacter rectus; Helicobacter pylori; Pseudomonas aeruginosa; Aggregatibacter actinomycetemcomitans; Phocaeicola vulgatus; Capnocytophaga gingivalis; Deinococcus radiodurans; Streptococcus mutans; Streptococcus intermedius; Cutibacterium acnes; Bacteria; Acinetobacter baumannii; Escherichia coli; Staphylococcus aureus; Staphylococcus epidermidis; Bacillus cereus; Schaalia odontolytica; Fusobacterium nucleatum subsp. nucleatum

Type:

Other

Platform:

GPL25612

178 Samples

Download data: CSV

(Submitter supplied) B. cereus RNA isolation were taken at OD 0.5 just before addition of 0.02 mg/mL EEC, and at 30 min of exposure. Water was added instead of EEC for control. Total RNA was isolated and we performed DNA microarray for genome-wide transcriptional analysis of B. cereus ATCC 14579 in the presence or absence of EEC. The microarray used in this study was custom-made B. cereus ATCC 14579 developed by Agilent Technologies (https://earray.chem.agilent.com/earray/). more...

Organism:

Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL20186

8 Samples

Download data: TXT

(Submitter supplied) Pathogenic species belonging to Bacillus cereus sensu lato group possess a high evolutionary advantage in the environment and in food matrices thanks to their capacity to survive as silent spores to harsh environmental insults and grow at relatively low temperatures. Ready to re-heat products are at severe risk for contamination by members of Bacillus cereus s.l. group if not stored at proper conditions. more...

Organism:

[Bacillus thuringiensis] serovar konkukian str. 97-27; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL19697

12 Samples

Download data: TXT

(Submitter supplied) Aims: To investigate the inactivation kinetics of Bacillus cereus vegetative cells upon exposure to low-temperature nitrogen gas plasma and to reveal the mode of inactivation by transcriptome profiling. Methods and results: Exponentially growing B. cereus cells were filtered and put on agar plates. The plates, carrying the filters with the vegetative cells, were placed into nitrogen gas plasma at atmospheric pressure and cold temperature (~37°C). more...

Organism:

Bacillus cereus; Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL9493

16 Samples

Download data: TXT

(Submitter supplied) Six bacterial genomes, Geobacter metallireducens GS-15, Chromohalobacter salexigens, Vibrio breoganii 1C-10, Bacillus cereus ATCC 10987, Campylobacter jejuni subsp. jejuni 81-176 and Campylobacter jejuni NCTC 11168, all of which had previously been sequenced using other platforms were re-sequenced using single-molecule, real-time (SMRT) sequencing specifically to analyze their methylomes. In every case a number of new N6-methyladenine (m6A) and N4-methylcytosine (m4C) methylation patterns were discovered and the DNA methyltransferases (MTases) responsible for those methylation patterns were assigned. more...

Organism:

Campylobacter jejuni; Geobacter metallireducens; Chromohalobacter israelensis; Bacillus cereus; Vibrio breoganii

Type:

Methylation profiling by high throughput sequencing

5 related Platforms

6 Samples

Download data: CSV, GFF

(Submitter supplied) Comparison of chitosan-treated B. cereus ATCC 14579 cells with non-treated B. cereus ATCC 14579 cells. 2 chitosans with similar molecular weight (Mw) but different degrees of acetylation (Fa) were used: chitosan B (Mw: 28.4 kDa, Fa: 0.16) (samples 1-3) and chitosan A (Mw: 36.0 kDa, Fa: 0.01) (samples 4-6).

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL13146

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL13146

9 Samples

Download data: TXT

(Submitter supplied) Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N)

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL13146

3 Samples

Download data: TXT

(Submitter supplied) Comparison of the Bacillus cereus with induced ComK1 (pATK31 in sample 1-3) or ComK2 (pATK32 in sample 4-6) proteins vs Bacillus cereus carrying empty plasmid (pLM5 in all samples)

Organism:

Bacillus cereus ATCC 14579; Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL13146

6 Samples

Download data: TXT

(Submitter supplied) We have employed a whole genome microarray for gene expression profilling to understand the global view of the cellular mechanism and metabolic response of B. cereus B3711 exposed to 1 mM silver nitrate. RNA was extracted from B. cereus grown under silver treated and untreated set of conditions in duplicates at two different time points (30 & 60 min). Comparing the transcript profile of the control and treated cells, several differentially expressed genes were identified. more...

Organism:

Bacillus cereus

Type:

Expression profiling by array

Platform:

GPL11312

8 Samples

Download data: TXT

(Submitter supplied) The goal of this study is the discovery of (a) meaningful phylogenomic relationships among members of this B. cereus/B. anthracis group, and (b) reliable gene-phenotype associations, e.g. recognition of links between genomic traits and the ability of certain strains to cause various forms of disease. We also tried to elucidate genome evolution aspects that may lead to the emergence of variants that are capable (or have the potential) of causing anthrax-like disease. more...

Organism:

Bacillus cereus; Bacillus anthracis; Bacillus cereus G9842; Bacillus anthracis str. Sterne

Type:

Genome variation profiling by array

Platforms:

GPL9098 GPL9095

315 Samples

Download data: MEV

(Submitter supplied) The goal of this project was to screen soil samples for bacteria that may harbor B. anthracis virulence-associated genes (VAGs). There is currently no information about the prevalence of these types of organisms in the environment. Due to increased environmental monitoring of select agents by programs such as BioWatch and biodetection systems in place at the United States Post Offices and Department of State locations, it has become critical that we not only better understand the natural range of B. more...

Organism:

Bacillus anthracis; Bacillus cereus; Bacillus anthracis str. Sterne

Type:

Genome variation profiling by array

Platforms:

GPL9098 GPL9095

202 Samples

Download data: MEV

(Submitter supplied) The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. more...

Organism:

Bacillus cereus; Bacillus anthracis

Type:

Genome variation profiling by array

Platform:

GPL9206

78 Samples

Download data: MEV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus cereus; Bacillus cereus ATCC 14579; Bacillus cereus ATCC 10987

Type:

Expression profiling by array

Platforms:

GPL7681 GPL7680

48 Samples

Download data