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| Status |
Public on May 12, 2015 |
| Title |
Global gene expression analysis of Bacillus cereus to proanthocyanidin exposure |
| Organism |
Bacillus cereus |
| Experiment type |
Expression profiling by array
|
| Summary |
B. cereus RNA isolation were taken at OD 0.5 just before addition of 0.02 mg/mL EEC, and at 30 min of exposure. Water was added instead of EEC for control. Total RNA was isolated and we performed DNA microarray for genome-wide transcriptional analysis of B. cereus ATCC 14579 in the presence or absence of EEC. The microarray used in this study was custom-made B. cereus ATCC 14579 developed by Agilent Technologies (https://earray.chem.agilent.com/earray/). The B. cereus microarray design was based on the predicted chromosomal open reading frames (NCBI Accession No. AE016877). It targets the 5234 annotated CDS, three non-overlapping probes were designed, low-quality probes were eliminated, and finally 15242 could be spotted. Microarray data was normalized to 75th percentile and the difference was found to be significant by unpaired T-test (P = 0.05). Next, ratio changes of 2-fold (for up-regulated genes in the EEC stress condition) and 0.5-fold (for down-regulated genes in the EEC stress condition) were regarded as biologically significant. 596 were up-regulated and 495 were down-regulated.
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| Overall design |
Bacillus cereus was selected at 0, 30 min exposure for RNA extraction and hybridization on Agilent microarrays.
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| Contributor(s) |
Tamura T, Mura K |
| Citation missing |
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| |
| Submission date |
May 11, 2015 |
| Last update date |
May 13, 2015 |
| Contact name |
Tomoko TAMURA |
| E-mail(s) |
t3tamura@nodai.ac.jp
|
| Organization name |
Tokyo University of Agriculture
|
| Department |
Nutritional Science and Food Safety
|
| Street address |
1-1-1 Sakuragaoka Setagaya-ku
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| City |
Tokyo |
| ZIP/Postal code |
156-8502 |
| Country |
Japan |
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| Platforms (1) |
| GPL20186 |
Agilent-034939 Bacillus_cereus_ATCC14579 [Probe Name Version] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA283794 |
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