|
| Status |
Public on Dec 04, 2012 |
| Title |
Tomato-2h_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Tomato-2h
|
| Organism |
Tetranychus urticae |
| Characteristics |
age: adult
|
| Growth protocol |
T. urticae strains, London, MAR-AB and MR-VP, were grown on potted kidney bean (Phaseolus vulgaris cv. "Prelude") plants at 26+-0.5°C and a 16/8h light/dark photoperiod. For the host change experiment, female adut mites from the London strain were grown for 2h, 12h, and 5 generations on tomato (Solanum lycopersicon cv. "Moneymaker") at 26°+-0.5°C and a 16/8 light/dark photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from about 100 adult female spider mites using RNEasy Mini Kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
100 ng of total RNA (+ RNA-spike in control) was used to generate Cy3 or Cy5 labeled cRNAs, using the Agilent Low Input Quick Amp Labeling Kit (version 6.5 Agilent Technologies)
|
| |
|
| Channel 2 |
| Source name |
London
|
| Organism |
Tetranychus urticae |
| Characteristics |
age: adult
|
| Growth protocol |
T. urticae strains, London, MAR-AB and MR-VP, were grown on potted kidney bean (Phaseolus vulgaris cv. "Prelude") plants at 26+-0.5°C and a 16/8h light/dark photoperiod. For the host change experiment, female adut mites from the London strain were grown for 2h, 12h, and 5 generations on tomato (Solanum lycopersicon cv. "Moneymaker") at 26°+-0.5°C and a 16/8 light/dark photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from about 100 adult female spider mites using RNEasy Mini Kit (Qiagen) following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA (+ RNA-spike in control) was used to generate Cy3 or Cy5 labeled cRNAs, using the Agilent Low Input Quick Amp Labeling Kit (version 6.5 Agilent Technologies)
|
| |
|
| |
| Hybridization protocol |
Cy3 and Cy5 labeled cRNAs were pooled and hybridized using the Gene Expression Hybridization Kit (Agilent Technologies) for 17h in a rotating hybridization oven at 20 r.p.m. and 65°C; Slides were washed using the Gene Expression Wash Buffer Kit (Agilent Technologies)
|
| Scan protocol |
Hybridized arrays were scanned using an Agilent Microarray High Resolution Scanner.
|
| Description |
Biological replicate 3 of 4, Tomato-2h vs. London
|
| Data processing |
Data were extracted and normalized using Agilent Feature Extraction Software 10.5 with default parameter settings for gene expression two-color microarrays (protocol GE2_105_Dec08). Further processed 'fold change' data are generated using Genespring.
|
| |
|
| Submission date |
Aug 03, 2012 |
| Last update date |
Dec 04, 2012 |
| Contact name |
Wannes Dermauw |
| E-mail(s) |
wannes.dermauw@ugent.be
|
| Phone |
003292646192
|
| Organization name |
University Ghent
|
| Department |
Crop Protection
|
| Lab |
Agrozoology
|
| Street address |
Coupure Links 653
|
| City |
Ghent |
| ZIP/Postal code |
9000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL15756 |
| Series (1) |
| GSE39869 |
A link between host plant adaptation and pesticide resistance in the polyphagous spider mite Tetranychus urticae |
|
