|
| Status |
Public on Aug 07, 2023 |
| Title |
hESCs cells, D45, replicate 3 |
| Sample type |
SRA |
| |
|
| Source name |
hESCs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hESCs
cell type: Human embryonic stem cells
time: Day45
|
| Treatment protocol |
To initiate differentiation, the E8 medium was changed to RPMI/B27 medium lacking insulin. CHIR99021 (6 μM; Selleckchem) was added to the cells from days 0 to 2, followed by the addition of IWR-1 (10 μM; Selleckchem) from days 3 to 5 for H7 cells. After day 7, cultures were maintained in RPMI/B27 medium containing insulin (Thermo Scientific), which was changed twice a week.
|
| Growth protocol |
Undifferentiated H7 ESCs were maintained on Matrigel (Thermo Scientific)-coated surfaces at 37°C and 5% CO2 in E8 medium plus supplements (Thermo Scientific)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy micro kit (Qiagen), according to the manufacturer’s protocol.
cDNA libraries were prepared by KAPA Stranded mRNA-Seq Kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Data processing |
Raw reads were aligned to hs37d5 using Tophat
Read count extraction and normalization were performed using htseq-count and Deseq-2
Assembly: hs37d5
Supplementary files format and content: tab-delimited files include raw counts for each sample
Supplementary files format and content: tab-delimited files include FPKMs for each sample
|
| |
|
| Submission date |
Aug 02, 2023 |
| Last update date |
Aug 07, 2023 |
| Contact name |
Ellen Ngar-Yun Poon |
| Organization name |
The Chinese University of Hong Kong
|
| Department |
School of Biomedical Sciences
|
| Street address |
Shatin
|
| City |
Hong Kong |
| State/province |
Hong Kong |
| ZIP/Postal code |
999077 |
| Country |
China |
| |
|
| Platform ID |
GPL18460 |
| Series (1) |
| GSE239918 |
Dynamic changes of mRNA expression during development of human embryonic stem cells derived cardiomyocytes |
|
| Relations |
| BioSample |
SAMN36811683 |
| SRA |
SRX21228790 |
