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Series GSE239918 Query DataSets for GSE239918
Status Public on Aug 07, 2023
Title Dynamic changes of mRNA expression during development of human embryonic stem cells derived cardiomyocytes
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To dissect complex regulatory mechanisms on cardiac development, we performed the time-series gene expression experiments of human embryonic stem cells (ESCs) differentiated to cardiomyocytes (CMs). The RNA-sequencing at 4 stages of human to CM maturation, representing timepoints with days 15, 30, 45 and 60 of ESC-CMs. The early CMs from differentiation days (D) 0-15 are commonly considered to be immature and embryonic-like. We define late CMs after D15. During this phase, the cells stop dividing and undergo some degree of maturation. This study established distinct dynamic expression patterns of mRNAs/genes and identified several subsets of up- or down-regulated genes with similar expression patterns during CM differentiation and maturation.
 
Overall design The RNA-sequencing of mRNA levels were conducted at 4 stages (15, 30, 45 and 60 days, +/- 2 days) of human H7 ESC-directed CM differentiation. Each stage included 3 biological replicates.
 
Contributor(s) Poon E, Boheler KR
Citation(s) 37705079
Submission date Aug 02, 2023
Last update date Nov 06, 2023
Contact name Ellen Ngar-Yun Poon
Organization name The Chinese University of Hong Kong
Department School of Biomedical Sciences
Street address Shatin
City Hong Kong
State/province Hong Kong
ZIP/Postal code 999077
Country China
 
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (12)
GSM7677388 hESCs cells, D15, replicate 1
GSM7677389 hESCs cells, D15, replicate 2
GSM7677390 hESCs cells, D15, replicate 3
Relations
BioProject PRJNA1001386

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE239918_RAW.tar 5.4 Mb (http)(custom) TAR (of TXT)
GSE239918_Raw_counts_allSamples.txt.gz 646.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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