|
Status |
Public on Sep 01, 2012 |
Title |
AC02-1382-01 |
Sample type |
RNA |
|
|
Source name |
Blood
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: ASPERGER'S DISORDER gender: male age at blood drawing (months): 192 race: Caucasian ethnicity: Non-Hispanic hours (minutes since last caloric intake): : 0:45 chronic diseases: - allergies: yes developmental/speech disorder: - neurological disorder: yes (positive lead screen) psychiatric disorder: yes (anxiety disorder, ADD) gastrointestinal disorder: - autoimmune disorder: - genetic testing: - birth defects: - ct: - mri: - medications/vitamin names: melatonin, multivitamins cerebral palsy: - seizure: - other diseases: -
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
Label |
biotin
|
Label protocol |
A total of 1 ug RNA was processed using established Affymetrix protocols for the generation of biotin-labeled cRNA.
|
|
|
Hybridization protocol |
The cRNA was quantified (using A260) and fragmented. Fragmented cRNA was hybridized on the GeneChips according to the manufacturer's standard protocol.
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 at 2.5 μm resolution.
|
Description |
Blood gene expression data from ASD
|
Data processing |
The data were analyzed with Probe Logarithmic Intensity Error (PLIER) estimation method using Affymetrix Power Tools version 1.10.
|
|
|
Submission date |
Jan 05, 2011 |
Last update date |
Sep 01, 2012 |
Contact name |
Sek Won Kong |
E-mail(s) |
swkong@enders.tch.harvard.edu
|
Phone |
617-919-2689
|
Organization name |
Boston Children's Hospital
|
Department |
Informatics Program
|
Lab |
EN137
|
Street address |
300 Longwood Avenue
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE18123 |
Blood gene expression signatures distinguish autism spectrum disorders from controls |
|