|
| Status |
Public on Sep 01, 2012 |
| Title |
AC05-1047-01 |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: AUTISM
gender: male
age at blood drawing (months): 100
race: Caucasian
ethnicity: Non-Hispanic
hours (minutes since last caloric intake): : unknown
chronic diseases: -
allergies: -
developmental/speech disorder: -
neurological disorder: -
psychiatric disorder: -
gastrointestinal disorder: -
autoimmune disorder: -
genetic testing: -
birth defects: -
ct: -
mri: -
medications/vitamin names: metadate, melatonin
cerebral palsy: -
seizure: -
other diseases: -
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
A total of 1 ug RNA was processed using established Affymetrix protocols for the generation of biotin-labeled cRNA.
|
| |
|
| Hybridization protocol |
The cRNA was quantified (using A260) and fragmented. Fragmented cRNA was hybridized on the GeneChips according to the manufacturer's standard protocol.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip scanner 3000 at 2.5 μm resolution.
|
| Description |
Blood gene expression data from ASD
|
| Data processing |
The data were analyzed with Probe Logarithmic Intensity Error (PLIER) estimation method using Affymetrix Power Tools version 1.10.
|
| |
|
| Submission date |
Jan 05, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Sek Won Kong |
| E-mail(s) |
swkong@enders.tch.harvard.edu
|
| Phone |
617-919-2689
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Lab |
EN137
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE18123 |
Blood gene expression signatures distinguish autism spectrum disorders from controls |
|
