|
| Status |
Public on Feb 02, 2019 |
| Title |
zmlrl5 knock down roothair RNA-seq, rep1 |
| Sample type |
SRA |
| |
|
| Source name |
roothair
|
| Organism |
Zea mays |
| Characteristics |
cultivar: W22 inbred line
developmental stage: 3-day old uniform
tissue: roothair
genotype: zmlrl5 knock down
|
| Treatment protocol |
3-day old uniform seedlings were dipped into liquid N and root hairs were scraped off and collected. The remaining root hair less roots and aerial seedling were collected
|
| Growth protocol |
seeds of the maize inbred line B73 were germinated in moist germination paper
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagenet(Invotrogen)
RNA-seq library construction was performed with NEB Next® mRNA Library Prep Master Mix Set (NEB, Ipswich, MA, USA) following manufacturer’s instructions
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Sequenced RNA-seq reads were trimmed to remove adaptor sequence and low-quality sequence ,then using Tophat2 and DESeq to identify differentially expressed genes.
Genome_build: B73 reference genome version 3
Supplementary_files_format_and_content: read count file was generated by HTSeq.
|
| |
|
| Submission date |
Nov 30, 2018 |
| Last update date |
Feb 02, 2019 |
| Contact name |
qi chuang ye |
| E-mail(s) |
qicy2014@cau.edu.cn
|
| Organization name |
china agriculture university
|
| Department |
national maize improvement center
|
| Lab |
Yan He
|
| Street address |
Yuanmingyuan West Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL17628 |
| Series (1) |
| GSE123164 |
Single-cell type transcriptome analysis of maize roothair |
|
| Relations |
| BioSample |
SAMN10503524 |
| SRA |
SRX5083754 |
