|
| Status |
Public on Feb 02, 2019 |
| Title |
Single-cell type transcriptome analysis of maize roothair |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated root hair-specific transcriptome using RNA-seq in maize. ZmLRL5 was further identified as a key regulator of maize root hair elongation.
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| |
|
| Overall design |
The maize inbred line B73 were germinated in moist germination paper. 3-day old uniform seedlings were dipped into liquid N and root hairs were scraped off and collected. The remaining root hair less roots were collected. From Total RNA was extracted using the Qiagen RNeasy Plant kit. Libraries were constructed and sequenced on an Illumina HiSeq2500
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| |
|
| Contributor(s) |
He Y |
| Citation(s) |
30556198 |
| Submission date |
Nov 30, 2018 |
| Last update date |
May 04, 2019 |
| Contact name |
qi chuang ye |
| E-mail(s) |
qicy2014@cau.edu.cn
|
| Organization name |
china agriculture university
|
| Department |
national maize improvement center
|
| Lab |
Yan He
|
| Street address |
Yuanmingyuan West Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platforms (1) |
|
| Samples (14)
|
| GSM3497815 |
root RNA-seq, rep2 |
| GSM3497816 |
aerial seedling RNA-seq, rep1 |
| GSM3497817 |
aerial seedling RNA-seq, rep2 |
| GSM3497818 |
wild type roothair RNA-seq, rep1 |
| GSM3497819 |
wild type roothair RNA-seq, rep2 |
| GSM3497820 |
zmlrl5 knock down roothair RNA-seq, rep1 |
| GSM3497821 |
zmlrl5 knock down roothair RNA-seq, rep2 |
| GSM3497822 |
wild type root (root hairs were scrapped off) RNA-seq, rep1 |
| GSM3497823 |
wild type root (root hairs were scrapped off) RNA-seq, rep2 |
| GSM3497824 |
zmlrl5 knock out root RNA-seq, rep1 |
| GSM3497825 |
zmlrl5 knock out root RNA-seq, rep2 |
|
| Relations |
| BioProject |
PRJNA507752 |
| SRA |
SRP171606 |
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