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Sample GSM3290432 Query DataSets for GSM3290432
Status Public on Nov 12, 2020
Title A42211
Sample type RNA
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Extramammary paget disease scrotum
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
VALUE The normalized value of 75 percentile (log2)

Data table
A_33_P3211513 1213.924
A_24_P278460 1164.878
A_21_P0014651 105.2279
A_23_P61637 2087.463
A_24_P120109 35.07655
A_23_P63896 1552.098634
A_24_P187056 1498.738
A_23_P122001 2745.204322
A_21_P0004300 369.1855
A_23_P88740 7525.832
A_23_P11652 9845.369934
A_23_P104025 1791.602926
A_23_P63459 9397.352
A_23_P252748 3052.483215
A_21_P0002212 581.972
A_21_P0004440 149.5091
A_23_P31073 5158.106
A_33_P3331085 402.0698
A_23_P123727 3997.800182
A_23_P109774 1500.790636

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.

Supplementary file Size Download File type/resource
GSM3290432_US10313827_253949448652_S01_GE1_1105_Oct12_2_2.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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