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Sample GSM3290431 Query DataSets for GSM3290431
Status Public on Nov 12, 2020
Title A0421202
Sample type RNA
Source name whole skin
Organism Homo sapiens
Characteristics tissue: Extramammary paget disease scrotum
gender: male
Extracted molecule total RNA
Extraction protocol RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
Label cy3
Label protocol cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
Hybridization protocol DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
Scan protocol Scanned on an Agilent G2565CA scanner.
Description Gene expression
Data processing Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
Submission date Jul 18, 2018
Last update date Nov 12, 2020
Contact name li na bu
Phone 18811357685
Organization name China Agricultural University
Street address NO.2 yuanmingyuan
City Beijing
ZIP/Postal code 100193
Country China
Platform ID GPL17077
Series (1)
GSE117285 Pattern of Extramammary Paget disease gene expression

Data table header descriptions
VALUE The normalized value of 75 percentile (log2)

Data table
A_33_P3211513 2185.636
A_24_P278460 1625.054
A_21_P0014651 132.3148
A_23_P61637 2867.293
A_24_P120109 27.06626
A_23_P63896 2489.84802
A_24_P187056 2782.221
A_23_P122001 3483.507635
A_21_P0004300 47.74503
A_23_P88740 4459.629
A_23_P11652 13357.16243
A_23_P104025 2058.294273
A_23_P63459 10008.99
A_23_P252748 4287.010155
A_21_P0002212 466.5725
A_21_P0004440 231.4227
A_23_P31073 4873.393
A_33_P3331085 366.9859
A_23_P123727 4245.744718
A_23_P109774 2366.30915

Total number of rows: 37480

Table truncated, full table size 819 Kbytes.

Supplementary file Size Download File type/resource
GSM3290431_US10313827_253949448652_S01_GE1_1105_Oct12_2_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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