|
| Status |
Public on Nov 12, 2020 |
| Title |
A0421202 |
| Sample type |
RNA |
| |
|
| Source name |
whole skin
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Extramammary paget disease scrotum
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
Gene expression
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Jul 18, 2018 |
| Last update date |
Nov 12, 2020 |
| Contact name |
li na bu |
| E-mail(s) |
linabu@cau.edu.cn
|
| Phone |
18811357685
|
| Organization name |
China Agricultural University
|
| Street address |
NO.2 yuanmingyuan
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE117285 |
Pattern of Extramammary Paget disease gene expression |
|
