|
| Status |
Public on Sep 05, 2017 |
| Title |
Ct-PHT57-VitD-100 |
| Sample type |
RNA |
| |
|
| Source name |
Isolated and in vitro cultured placental cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: cytotrophoblasts
treatment: 100 nM 1,25(OH)2D3
|
| Treatment protocol |
Primary cultures (n = 6) of human trophoblast from term placentas, with phenotypes previously characterized in our lab (Wang Reproduction 1521: 97). Ethanol control or 100 nM 1,25(OH)2D were added for the final 6 h
|
| Growth protocol |
Primary human villous cytotrophoblasts were isolated as described {Chen et al., 2010, Am J Physiol Cell Physiol, 299, C968-76; Chen et al., 2015, Placenta, 36, 847-53} and washed thoroughly 4 h after plating, to eliminate villous fragments and nonviable cells. Cytotrophoblasts were cultured for a total time of 24 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation with TRI REAGENT according to manufacturer
|
| Label |
biotin
|
| Label protocol |
The Ambion® WT Expression Kit; For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 09/2009 Rev C (Cat No. 4411973 ), Affymetrix WT Terminal Labeling Kit 30rxn (Cat No. 900671)
|
| |
|
| Hybridization protocol |
GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix Genechip® fluidics station 450: protocol FS450_0007 for Cartridge Arrays
|
| Scan protocol |
Affymetrix Scanner: GCS3000; AGCC (Command Console Software AGCC_3_1_1.)
|
| Description |
total RNA
|
| Data processing |
Geneexpression Console v.1.1, Affymetrix (Expression Console EC_1_1_2) using Affymetrix default analysis settings for generation of CEL-files.
Data Pre-Processing (Partek Software, v.6.5: RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization); Biostatistical data analysis:
|
| |
|
| Submission date |
Feb 28, 2017 |
| Last update date |
Sep 05, 2017 |
| Contact name |
Silvija Tokic |
| E-mail(s) |
silvija.tokic@medunigraz.at
|
| Organization name |
Medical University of Graz
|
| Street address |
Auenbruggerplatz 14
|
| City |
Graz |
| ZIP/Postal code |
8036 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE95497 |
Vitamin D regulates expression of multiple genes including CD14 and CD180 to modulate the syncytiotrophoblast response to lipopolysaccharide |
|
